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MSTI Fluorescence High-Throughput Drug Assay

Info

Catalogue Number 153815
Type Fluorescent Probe
Relevance This novel fluorescence-based high throughput assay allows for the detection of thiol-reactive drug candidates that are likely to irreversibly interact with biological targets. These promiscuous inhibitors can be identified rapidly, in parallel, for small molecule screening libraries using 384 or 1536 well plate formats. Testing small molecules for their ability to modulate cysteine residues of proteins in the early stages of drug discovery is expected to increase efficiency and success of every HTS campaign.
Molecular Formula C19H20NS
lUPAC Acetyl-(E)-2-(4-mercaptostyryl)-1,3,3-trimethyl-3H-indol-1-ium
Molecular Weight (g/mol) 294.1
Solubility DMSO, DMF, methanol, water (partially)
Research Area Drug Discovery & Development
Storage -20C, protect from light
Notes More accurate – Less interference from molecules due to use of far red spectrum
Faster – Can be used for high throughput screening (1536-well plate format)
More versatile – Identifies both electrophilic and redox reactive compounds
Stable product – Use of acetylated precursor allows for storage of the assay probe and its
reliable generation in situ

References

There are 2 reference entries for this reagent.

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References: 2 entries

McCallum et al. 2013. J Biomol Screen. 18(6):705-13. PMID: 23446699.

High-throughput identification of promiscuous inhibitors from screening libraries with the use of a thiol-containing fluorescent probe.

Europe PMC ID: 23446699


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References: 2 entries

McCallum et al. 2013. J Biomol Screen. 18(6):705-13. PMID: 23446699.

High-throughput identification of promiscuous inhibitors from screening libraries with the use of a thiol-containing fluorescent probe.


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