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Catalogue Number 153794
Backbone Size (bp) 2958
Bacterial Resistance Ampicillin
Vector Type pBluescript KS
Synonyms Cystic Fibrosis Transmembrane Conductance Regulator, Channel Conductance-Controlling ATPase
Antigen/Gene or Protein Targets CFTR Exon 9 minigene
Relevance This minigene construct consists of a minimal α-globin promoter and SV40 enhancer which drive the transcription of the minigene. Downstream, an alpha-globin-fibronectin EDB minigene is present with a unique NdeI site where a fragment that contains exon 9 (183bp) along with part of the flanking introns was inserted. The exon 9 sequence also carries a C155T mutation that destroys a splicing enhancer within its sequence. This means that when transfected into cells the exon 9 is included approximately in 50% of the transcripts. In this manner, it is possible to see changes both with regards to upregulation of exon inclusion or its downregulation.
Research Area Gene Expression
Notes Concentration 2mg/ml

References

There are 4 reference entries for this reagent.

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References: 4 entries
RECENTLY UPDATED

D'Ambrogio et al. 2009. Nucleic Acids Res. 37(12):4116-26. PMID: 19429692.

Functional mapping of the interaction between TDP-43 and hnRNP A2 in vivo.

Europe PMC ID: 19429692

Pagani et al. 2003. J Biol Chem. 278(29):26580-8. PMID: 12732620.

Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9.

Europe PMC ID: 12732620


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References: 4 entries
RECENTLY UPDATED

D'Ambrogio et al. 2009. Nucleic Acids Res. 37(12):4116-26. PMID: 19429692.

Functional mapping of the interaction between TDP-43 and hnRNP A2 in vivo.

Pagani et al. 2003. J Biol Chem. 278(29):26580-8. PMID: 12732620.

Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9.


Add a reference