pTB_CFTR_Ex9 155T vector
Invented by Prof Emanuele Buratti from International Centre For Genetic Engineering And Biotechnology (ICGEB)
Invented at International Centre For Genetic Engineering And Biotechnology (ICGEB)
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 153794 |
| Backbone Size (bp) | 2958 |
| Bacterial Resistance | Ampicillin |
| Vector Type | pBluescript KS |
| Synonyms | Cystic Fibrosis Transmembrane Conductance Regulator, Channel Conductance-Controlling ATPase |
| Antigen/Gene or Protein Targets | CFTR Exon 9 minigene |
| Relevance | This minigene construct consists of a minimal α-globin promoter and SV40 enhancer which drive the transcription of the minigene. Downstream, an alpha-globin-fibronectin EDB minigene is present with a unique NdeI site where a fragment that contains exon 9 (183bp) along with part of the flanking introns was inserted. The exon 9 sequence also carries a C155T mutation that destroys a splicing enhancer within its sequence. This means that when transfected into cells the exon 9 is included approximately in 50% of the transcripts. In this manner, it is possible to see changes both with regards to upregulation of exon inclusion or its downregulation. |
| Research Area | Gene Expression |
| Notes | Concentration 2mg/ml |
References: 4 entries
D'Ambrogio et al. 2009. Nucleic Acids Res. 37(12):4116-26. PMID: 19429692.
Functional mapping of the interaction between TDP-43 and hnRNP A2 in vivo.
Europe PMC ID: 19429692
Pagani et al. 2003. J Biol Chem. 278(29):26580-8. PMID: 12732620.
Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9.
Europe PMC ID: 12732620
Add a reference
References: 4 entries
D'Ambrogio et al. 2009. Nucleic Acids Res. 37(12):4116-26. PMID: 19429692.
Functional mapping of the interaction between TDP-43 and hnRNP A2 in vivo.
Pagani et al. 2003. J Biol Chem. 278(29):26580-8. PMID: 12732620.
Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9.
Add a reference
