Cancer Research Technology
Log in Register
Menu

Anti-epidermis-type lipoxygenase 3 (Aloxe3), Polyclonal

Invented by Dr Peter Krieg at Deutsches Krebsforschungszentrum (DKFZ) German Cancer Research Center

Info

Catalogue Number 153777
Applications IP WB
Antigen/Gene or Protein Targets Aloxe3
Synonyms Hydroperoxide isomerase ALOXE3, Epidermis-type lipoxygenase 3, Epidermal LOX-3, e-LOX-3, eLOX-3, Hydroperoxy icosatetraenoate dehydratase
Reactivity Human and Mouse
Relevance Non-heme iron-containing lipoxygenase which is atypical in that it displays a prominent hydroperoxide isomerase activity and a reduced dioxygenase activity compared to other lipoxygenases. The hydroperoxide isomerase activity catalyzes the isomerization of hydroperoxides, derived from arachidonic and linoleic acid by ALOX12B, into hepoxilin-type epoxyalcohols. The dioxygenase activity requires a step of activation of the enzyme by molecular oxygen. In presence of oxygen, oxygenates polyunsaturated fatty acids, including arachidonic acid, to produce fatty acid hydroperoxides. In the skin, acts downstream of ALOX12B on the linoleate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. In parallel, it may have a signaling function in barrier formation through the production of hepoxilins metabolites. Plays also a role in adipocyte differentiation through hepoxilin A3 and hepoxilin B3 production which in turn activate PPARG. Through the production of hepoxilins in the spinal cord, it may regulate inflammatory tactile allodynia.
Host Rabbit
Immunogen Mouse epidermis-type lipoxygenase-3 (Y14695) Peptide Z1: aa 167-189 SDKKFALTKTVPCAEQDDNSGNR
Formulation Neat sera
Notes Western blot dilution: 1:5,000
Research Area Adhesion, Cell Structure and Motility, Metabolism

References

There are 0 reference entries for this reagent.

References: 0 entry

There is no reference for this reagent yet, feel free to use the button below to suggest one.

Add a reference

References: 0 entry

There is no reference for this reagent yet, feel free to use the button below to suggest one.

Add a reference