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THCECb Cell Line

Invented by Prof Michael Apicella at The University Of Iowa

Info

Catalogue Number 153755
Antigen/Gene or Protein Targets Sexually transmitted diseases (STIs)
Host Human
Tissue Cervix
Disease Keywords Neisseria gonorrhoeae infection, Gonorrhea
Model Immortalised Line
Relevance This cell line was developed to study Neisseria gonorrhoeae (Gonorrhea) infection in women. Women with N. gonorrhoeae are often asymptomatic leading to chronic infections, upper genital tract infections leading to pelvic inflammatory disease, and increase risk for contracting HIV. This cell line has been used in studying biofilm production of N. gonorrhoeae which can exacerbate the illness and infection.

This cell line expresses complement receptor type III on the surface and secretes complement components, necessary for gonococcal attachment and invasion of cervical epithelial cells.
Production Details This cell line was derived from primary cervical cells obtained from cervical biopsies and immortalized with E6/E7.
Conditional No
Research Area Immunology
Recommended Growing Conditions Serum-free keratinocyte growth media (K-SFM) supplement 500 mL of basal media with 12.5 mg BPE and 0.08 ug EGF. Media may be supplemented with 15 PenStrep for routine maintenance.

References

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References: 1 entry

Falsetta et al. 2009. Infect Immun. 77(9):3522-32. PMID: 19528210.


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References: 1 entry

Falsetta et al. 2009. Infect Immun. 77(9):3522-32. PMID: 19528210.


Add a reference