Cancer Research Technology
Log in Register
Menu

16E6-E7 HFK
RECENTLY UPDATED

Info

Catalogue Number 153752
Antigen/Gene or Protein Targets Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies
Parental Line
RECENTLY UPDATED
Human foreskin keratinocytes (HFKs) established from foreskin tissue obtained from newborns
Host
RECENTLY UPDATED
Human
Relevance Expresses skin cell markers; proinflammatory
Production Details
RECENTLY UPDATED
HPV-16 E7 expressing keratinocytes were generated by infecting normal primary cells with an LXSH retrovirus that expressed E7 and conferred hygromycin resistance. After selection in hygromycin, cells were infected with an LXSN retroviral construct that expressed wild-type HPV-16 E6 and conferred G418 resistance. 
Conditional No
Research Area Cancer, Microbiology, Virology
Recommended Growing Conditions
RECENTLY UPDATED
Maintained in Keratinocyte-SFM (Gibco-BRL, Rockville, MD). G418 selection.
Notes Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies

References

There are 1 reference entries for this reagent.

View All References

References: 1 entry
RECENTLY UPDATED

Duffy et al. 2003. Virology. 314(1):196-205. PMID: 14517073.


Add a reference

References: 1 entry
RECENTLY UPDATED

Duffy et al. 2003. Virology. 314(1):196-205. PMID: 14517073.


Add a reference

Inventor Information

No inventors are currently linked to this reagent.

Add an inventor