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Catalogue Number
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153752 |
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Antigen/Gene or Protein Targets
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Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies
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Parental Line
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Human foreskin keratinocytes (HFKs) established from foreskin tissue obtained from newborns
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Host
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Human
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Disease Keywords
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Cancer
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Model
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Immortalised Line
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Relevance
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Expresses skin cell markers; proinflammatory
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Production Details
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HPV-16 E7 expressing keratinocytes were generated by infecting normal primary cells with an LXSH retrovirus that expressed E7 and conferred hygromycin resistance. After selection in hygromycin, cells were infected with an LXSN retroviral construct that expressed wild-type HPV-16 E6 and conferred G418 resistance.
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Conditional
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No
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Research Area
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Cancer, Microbiology, Virology
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Recommended Growing Conditions
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Maintained in Keratinocyte-SFM (Gibco-BRL, Rockville, MD). G418 selection.
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Notes
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Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies
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