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16E6-E7 HFK

Info

Catalogue Number 153752
Antigen/Gene or Protein Targets Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies
Parental Line Human foreskin keratinocytes (HFKs) established from foreskin tissue obtained from newborns
Host Human
Relevance Expresses skin cell markers; proinflammatory
Production Details HPV-16 E7 expressing keratinocytes were generated by infecting normal primary cells with an LXSH retrovirus that expressed E7 and conferred hygromycin resistance. After selection in hygromycin, cells were infected with an LXSN retroviral construct that expressed wild-type HPV-16 E6 and conferred G418 resistance. 
Conditional No
Research Area Cancer, Microbiology, Virology
Recommended Growing Conditions Maintained in Keratinocyte-SFM (Gibco-BRL, Rockville, MD). G418 selection.
Notes Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies

References

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References: 1 entry

Duffy et al. 2003. Virology. 314(1):196-205. PMID: 14517073.


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References: 1 entry

Duffy et al. 2003. Virology. 314(1):196-205. PMID: 14517073.


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