Cancer Research Technology
Log in Register
Menu

Human AGS NOD1 KO (Clone 41A8) Cell Line

Info

Catalogue Number 153733
Antigen/Gene or Protein Targets Human nucleotide-binding oligomerization domain-containing protein 1
Parental Line AGS
Synonyms Human nucleotide-binding oligomerization domain-containing protein 1, NOD1.
Host Human
Tissue Gastric
Disease Keywords Gastric Adenocarcinoma
Model Knock-Out
Relevance NOD1 responds to Helicobacter pylori infection in a Type IV secretion system-dependent manner. H. pylori causes chronic inflammation, a key precursor to gastric adenocarcinoma. AGS cells deficient in NOD1 can be used to model gastric adenocarcinoma in the context of various pathogens. NOD1 regulates host epithelial cell responses. Upregulates antimicrobial peptides of the beta-defensin (hBD) family secreted by epithelial cells.
Production Details CRISPR/Cas9 gene editing was used to generate NOD1 knock out clones. AGS cells were transfected with vectors expressing “Cas9 nickase” nuclease and pairs of guide RNAs (gRNAs) specific for the NOD1 gene. Clone 41A8 was confirmed as having a 71 base pair deletion within the coding regions of the caspase activation and recruitment domain (CARD) of NOD1.
Conditional No
Research Area Cancer, Cell Signaling & Signal Transduction, Immunology
Growth/Phenotype Keywords Epithelial-like morphology
Recommended Growing Conditions RPMI with: 10% foetal calf serum; 1% penicillin-streptomycin; and 1% L-glutamine. Subculture cells every 2-3 days in ratios of 1:2-1:4 in T25 or T75 flasks.
Positive Control NOD1 Ligand: C12-iE-DAP (10 ng/ml)
Notes Recommended Freezing Composition: 90% (v/v) FCS - 10% (v/v) DMSO

Sold for academic research use.If reagent is required for commercialisation purposes, contact Ximbio at enquiries@ximbio.com.

The control cell line Human AGS Control Cell Line can be purchased from ATCC http://www.lgcstandards-atcc.org/products/all/CRL-1739.aspx?geo_country=gb.

References

There are 2 reference entries for this reagent.

View All References

References: 2 entries

Tran et al. 2018. Cell Microbiol. 20(5):e12826. PMID: 29392836.

NOD1 is required for Helicobacter pylori induction of IL-33 responses in gastric epithelial cells.

Europe PMC ID: 29392836


Add a reference

References: 2 entries

Tran et al. 2018. Cell Microbiol. 20(5):e12826. PMID: 29392836.

NOD1 is required for Helicobacter pylori induction of IL-33 responses in gastric epithelial cells.


Add a reference

Inventor Information

No inventors are currently linked to this reagent.

Add an inventor