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Anti-Cytochrome P450 1B1 [5D3]

Invented by Ayham Alnabulsi at Vertebrate Antibodies Limited

Info

Catalogue Number 153656
Applications ELISA IHC WB
Antigen/Gene or Protein Targets Human cytochrome P450 1B1
Synonyms Aryl hydrocarbon hydroxylase antibody, CP1B antibody, CP1B1_HUMAN antibody, Cyp1b1 antibody, CYPIB1 antibody, Cytochrome P450 1B1 antibody, Cytochrome P450 family 1 subfamily B polypeptide 1 antibody, Cytochrome P450 subfamily I (dioxin inducible) polypeptide 1 (glaucoma 3 primary infantile) antibody, Flavoprotein linked monooxygenase antibody, GLC3A antibody, Microsomal monooxygenase antibody, P4501B1 antibody, Xenobiotic monooxygenase antibody
Reactivity Human
Relevance Monoclonal antibody directed against CYP1B1, a possible anti-cancer target.

Background and Research Application
Cytochrome P450 (CYP) 1B1 is overexpressed in tumour cells and is recognised as a biomarker of the tumour phenotype. Cytochrome P450 1B1 (CYP1B1) is active in the metabolism of oestrogens to reactive catechols and of different procarcinogens. The cytochrome P450 proteins are monooxygenases which catalyse many reactions involved in drug metabolism and the synthesis of cholesterol, steroids and other lipids.
Recent studies have highlighted the potential of this enzyme as a novel cancer therapeutic target, resulting in several clinical development programs focused on CYP1B1-activated prodrugs and CYP1B1 inhibitors.
Host Mouse
Immunogen Ovalbumin-conjugated synthetic peptide; PENFDPARFLDKDGL (aa. 437-451). Peptide immunogen is identical in mouse (Mus musculus), rat (Rattus norvegicus), dog (Canis familiaris), pig (Sus scrofa) and horse (Equus caballus).
Immunogen UniProt ID Q16678
Positive Control IHC: formalin-fixed, paraffin-embedded ovarian cancer sections; Western Blot: rhCYP1B1 from lymphoblastoid cells (Gentest); 10mcg microsomal protein / lane
Subclass IgG1 kappa
Myeloma Used P3X63Ag8.653
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
The majority of breast cancers showed positive immunoreactivity for CYP1B1, and in each case CYP1B1 was specifically localised to tumour cells.

Not reactive with CYP1A1. IHC antigen retrieval: microwave 20 min @ 800W in 10mM citrate buffer, pH 6.0.

Concentration
1mg/ml as standard
Research Area Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Metabolism, Neurobiology

References

There are 10 reference entries for this reagent.

View All References

References: 10 entries

Carnell et al. 2004. Int J Radiat Oncol Biol Phys. 58(2):500-9. PMID: 14751521.

Target validation of cytochrome P450 CYP1B1 in prostate carcinoma with protein expression in associated hyperplastic and premalignant tissue.

Europe PMC ID: 14751521

Maecker et al. 2003. Blood. 102(9):3287-94. PMID: 12869499.

The shared tumor-associated antigen cytochrome P450 1B1 is recognized by specific cytotoxic T cells.

Europe PMC ID: 12869499

McFadyen et al. 2001. Br J Cancer. 85(2):242-6. PMID: 11461084.

IHC

Cytochrome P450 CYP1B1 over-expression in primary and metastatic ovarian cancer.

Europe PMC ID: 11461084

Murray et al. 2001. Annu Rev Pharmacol Toxicol. 41:297-316. PMID: 11264459.

IHC

Regulation, function, and tissue-specific expression of cytochrome P450 CYP1B1.

Europe PMC ID: 11264459

McFadyen et al. 1999. J Histochem Cytochem. 47(11):1457-64. PMID: 10544218.

IHC

Immunohistochemical localization of cytochrome P450 CYP1B1 in breast cancer with monoclonal antibodies specific for CYP1B1.

Europe PMC ID: 10544218


Add a reference

References: 10 entries

Carnell et al. 2004. Int J Radiat Oncol Biol Phys. 58(2):500-9. PMID: 14751521.

Target validation of cytochrome P450 CYP1B1 in prostate carcinoma with protein expression in associated hyperplastic and premalignant tissue.

Maecker et al. 2003. Blood. 102(9):3287-94. PMID: 12869499.

The shared tumor-associated antigen cytochrome P450 1B1 is recognized by specific cytotoxic T cells.

McFadyen et al. 2001. Br J Cancer. 85(2):242-6. PMID: 11461084.

IHC

Cytochrome P450 CYP1B1 over-expression in primary and metastatic ovarian cancer.

Murray et al. 2001. Annu Rev Pharmacol Toxicol. 41:297-316. PMID: 11264459.

IHC

Regulation, function, and tissue-specific expression of cytochrome P450 CYP1B1.

McFadyen et al. 1999. J Histochem Cytochem. 47(11):1457-64. PMID: 10544218.

IHC

Immunohistochemical localization of cytochrome P450 CYP1B1 in breast cancer with monoclonal antibodies specific for CYP1B1.


Add a reference