Anti-Cytochrome P450 1B1 [5D3]
Invented by Ayham Alnabulsi at Vertebrate Antibodies Limited
- Datasheet
- References (10)
- Inventor Info
Info
![Image for Anti-Cytochrome P450 1B1 [5D3]](https://res.cloudinary.com/ximbio/image/upload/c_fit/2c7ec30a-5231-4619-ac40-f997a74c27d4.PNG)
![Immunohistochemistry images showing staining of formalin-fixed, paraffin-embedded human tissue sections using Anti-Cytochrome P450 1B1 [5D3]](https://res.cloudinary.com/ximbio/image/upload/c_fit/7add464d-6991-4726-b642-f315f6c570e1.png)
![Western blot using Anti-Cytochrome P450 1B1 [5D3]. (1) microsomes from human lymphoblastoid cells expressing rhCYP1B1, (2) microsomes from human lymphoblastoid cells expressing rhCYP1A1, (3) expression vector control, (4) normal human liver microsomes (10mcg microsomal protein/lane, all from Gentest Corp).](https://res.cloudinary.com/ximbio/image/upload/c_fit/ed8150c0-615a-4348-af7c-9f4c338936a4.png)
![Determination LOD in western blot of and Anti-Cytochrome P450 1B1 [5D3] using Gentest rhCYP1B1 microsomes.](https://res.cloudinary.com/ximbio/image/upload/c_fit/48c30162-f6d9-408d-8381-0bc49435ee36.png)
![Image for Anti-Cytochrome P450 1B1 [5D3]](https://res.cloudinary.com/ximbio/image/upload/c_limit,h_282,w_368/2c7ec30a-5231-4619-ac40-f997a74c27d4.PNG)
![Immunohistochemistry images showing staining of formalin-fixed, paraffin-embedded human tissue sections using Anti-Cytochrome P450 1B1 [5D3]](https://res.cloudinary.com/ximbio/image/upload/c_limit,h_282,w_368/7add464d-6991-4726-b642-f315f6c570e1.png)
![Western blot using Anti-Cytochrome P450 1B1 [5D3]. (1) microsomes from human lymphoblastoid cells expressing rhCYP1B1, (2) microsomes from human lymphoblastoid cells expressing rhCYP1A1, (3) expression vector control, (4) normal human liver microsomes (10mcg microsomal protein/lane, all from Gentest Corp).](https://res.cloudinary.com/ximbio/image/upload/c_limit,h_282,w_368/ed8150c0-615a-4348-af7c-9f4c338936a4.png)
![Determination LOD in western blot of and Anti-Cytochrome P450 1B1 [5D3] using Gentest rhCYP1B1 microsomes.](https://res.cloudinary.com/ximbio/image/upload/c_limit,h_282,w_368/48c30162-f6d9-408d-8381-0bc49435ee36.png)
![Image thumbnail for Anti-Cytochrome P450 1B1 [5D3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,h_45/2c7ec30a-5231-4619-ac40-f997a74c27d4.PNG)
![Image thumbnail for Anti-Cytochrome P450 1B1 [5D3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,h_45/7add464d-6991-4726-b642-f315f6c570e1.png)
![Image thumbnail for Anti-Cytochrome P450 1B1 [5D3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,h_45/ed8150c0-615a-4348-af7c-9f4c338936a4.png)
![Image thumbnail for Anti-Cytochrome P450 1B1 [5D3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,h_45/48c30162-f6d9-408d-8381-0bc49435ee36.png)
| Catalogue Number | 153656 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Human cytochrome P450 1B1 |
| Synonyms | Aryl hydrocarbon hydroxylase antibody, CP1B antibody, CP1B1_HUMAN antibody, Cyp1b1 antibody, CYPIB1 antibody, Cytochrome P450 1B1 antibody, Cytochrome P450 family 1 subfamily B polypeptide 1 antibody, Cytochrome P450 subfamily I (dioxin inducible) polypeptide 1 (glaucoma 3 primary infantile) antibody, Flavoprotein linked monooxygenase antibody, GLC3A antibody, Microsomal monooxygenase antibody, P4501B1 antibody, Xenobiotic monooxygenase antibody |
| Reactivity | Human |
| Relevance | Cytochrome P450 (CYP) 1B1 is overexpressed in tumour cells and is recognised as a biomarker of the tumour phenotype. Recent studies have highlighted the potential of this enzyme as a novel cancer therapeutic target, resulting in several clinical development programs focused on CYP1B1-activated prodrugs and CYP1B1 inhibitors. |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptide; PENFDPARFLDKDGL (aa. 437-451). Peptide immunogen is identical in mouse (Mus musculus), rat (Rattus norvegicus), dog (Canis familiaris), pig (Sus scrofa) and horse (Equus caballus). |
| Positive Control | IHC: formalin-fixed, paraffin-embedded ovarian cancer sections; Western Blot: rhCYP1B1 from lymphoblastoid cells (Gentest); 10mcg microsomal protein / lane |
| Subclass | IgG1 kappa |
| Myeloma Used | P3X63Ag8.653 |
| Strain | Balb/c |
| Notes | Not reactive with CYP1A1. IHC antigen retrieval: microwave 20 min @ 800W in 10mM citrate buffer, pH 6.0. |
| Research Area | Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Metabolism, Neurobiology |
References: 10 entries
Carnell et al. 2004. Int J Radiat Oncol Biol Phys. 58(2):500-9. PMID: 14751521.
Target validation of cytochrome P450 CYP1B1 in prostate carcinoma with protein expression in associated hyperplastic and premalignant tissue.
Europe PMC ID: 14751521
Maecker et al. 2003. Blood. 102(9):3287-94. PMID: 12869499.
The shared tumor-associated antigen cytochrome P450 1B1 is recognized by specific cytotoxic T cells.
Europe PMC ID: 12869499
McFadyen et al. 2001. Br J Cancer. 85(2):242-6. PMID: 11461084.
IHC
Cytochrome P450 CYP1B1 over-expression in primary and metastatic ovarian cancer.
Europe PMC ID: 11461084
Murray et al. 2001. Annu Rev Pharmacol Toxicol. 41:297-316. PMID: 11264459.
IHC
Regulation, function, and tissue-specific expression of cytochrome P450 CYP1B1.
Europe PMC ID: 11264459
McFadyen et al. 1999. J Histochem Cytochem. 47(11):1457-64. PMID: 10544218.
IHC
Immunohistochemical localization of cytochrome P450 CYP1B1 in breast cancer with monoclonal antibodies specific for CYP1B1.
Europe PMC ID: 10544218
Add a reference
References: 10 entries
Carnell et al. 2004. Int J Radiat Oncol Biol Phys. 58(2):500-9. PMID: 14751521.
Target validation of cytochrome P450 CYP1B1 in prostate carcinoma with protein expression in associated hyperplastic and premalignant tissue.
Maecker et al. 2003. Blood. 102(9):3287-94. PMID: 12869499.
The shared tumor-associated antigen cytochrome P450 1B1 is recognized by specific cytotoxic T cells.
McFadyen et al. 2001. Br J Cancer. 85(2):242-6. PMID: 11461084.
IHC
Cytochrome P450 CYP1B1 over-expression in primary and metastatic ovarian cancer.
Murray et al. 2001. Annu Rev Pharmacol Toxicol. 41:297-316. PMID: 11264459.
IHC
Regulation, function, and tissue-specific expression of cytochrome P450 CYP1B1.
McFadyen et al. 1999. J Histochem Cytochem. 47(11):1457-64. PMID: 10544218.
IHC
Immunohistochemical localization of cytochrome P450 CYP1B1 in breast cancer with monoclonal antibodies specific for CYP1B1.
Add a reference
