Anti-Cytochrome P450 1B1 [5D3]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
- Datasheet
- References (10)
- Inventor Info
Info
![Image for Anti-Cytochrome P450 1B1 [5D3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/2c7ec30a-5231-4619-ac40-f997a74c27d4.PNG)
![Immunohistochemistry images showing staining of formalin-fixed, paraffin-embedded human tissue sections using Anti-Cytochrome P450 1B1 [5D3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/7add464d-6991-4726-b642-f315f6c570e1.png)
![Western blot using Anti-Cytochrome P450 1B1 [5D3]. (1) microsomes from human lymphoblastoid cells expressing rhCYP1B1, (2) microsomes from human lymphoblastoid cells expressing rhCYP1A1, (3) expression vector control, (4) normal human liver microsomes (10mcg microsomal protein/lane, all from Gentest Corp).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/ed8150c0-615a-4348-af7c-9f4c338936a4.png)
![Determination LOD in western blot of and Anti-Cytochrome P450 1B1 [5D3] using Gentest rhCYP1B1 microsomes.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/48c30162-f6d9-408d-8381-0bc49435ee36.png)
![Image for Anti-Cytochrome P450 1B1 [5D3]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/2c7ec30a-5231-4619-ac40-f997a74c27d4.PNG)
![Immunohistochemistry images showing staining of formalin-fixed, paraffin-embedded human tissue sections using Anti-Cytochrome P450 1B1 [5D3]](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/7add464d-6991-4726-b642-f315f6c570e1.png)
![Western blot using Anti-Cytochrome P450 1B1 [5D3]. (1) microsomes from human lymphoblastoid cells expressing rhCYP1B1, (2) microsomes from human lymphoblastoid cells expressing rhCYP1A1, (3) expression vector control, (4) normal human liver microsomes (10mcg microsomal protein/lane, all from Gentest Corp).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/ed8150c0-615a-4348-af7c-9f4c338936a4.png)
![Determination LOD in western blot of and Anti-Cytochrome P450 1B1 [5D3] using Gentest rhCYP1B1 microsomes.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/48c30162-f6d9-408d-8381-0bc49435ee36.png)
![Image thumbnail for Anti-Cytochrome P450 1B1 [5D3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/2c7ec30a-5231-4619-ac40-f997a74c27d4.PNG)
![Image thumbnail for Anti-Cytochrome P450 1B1 [5D3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/7add464d-6991-4726-b642-f315f6c570e1.png)
![Image thumbnail for Anti-Cytochrome P450 1B1 [5D3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/ed8150c0-615a-4348-af7c-9f4c338936a4.png)
![Image thumbnail for Anti-Cytochrome P450 1B1 [5D3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/48c30162-f6d9-408d-8381-0bc49435ee36.png)
| Catalogue Number | 153656 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Human cytochrome P450 1B1 |
| Synonyms | Aryl hydrocarbon hydroxylase antibody, CP1B antibody, CP1B1_HUMAN antibody, Cyp1b1 antibody, CYPIB1 antibody, Cytochrome P450 1B1 antibody, Cytochrome P450 family 1 subfamily B polypeptide 1 antibody, Cytochrome P450 subfamily I (dioxin inducible) polypeptide 1 (glaucoma 3 primary infantile) antibody, Flavoprotein linked monooxygenase antibody, GLC3A antibody, Microsomal monooxygenase antibody, P4501B1 antibody, Xenobiotic monooxygenase antibody |
| Reactivity | Human |
| Relevance |
Monoclonal antibody directed against CYP1B1, a possible anti-cancer target. Background and Research Application Cytochrome P450 (CYP) 1B1 is overexpressed in tumour cells and is recognised as a biomarker of the tumour phenotype. Cytochrome P450 1B1 (CYP1B1) is active in the metabolism of oestrogens to reactive catechols and of different procarcinogens. The cytochrome P450 proteins are monooxygenases which catalyse many reactions involved in drug metabolism and the synthesis of cholesterol, steroids and other lipids. Recent studies have highlighted the potential of this enzyme as a novel cancer therapeutic target, resulting in several clinical development programs focused on CYP1B1-activated prodrugs and CYP1B1 inhibitors. |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptide; PENFDPARFLDKDGL (aa. 437-451). Peptide immunogen is identical in mouse (Mus musculus), rat (Rattus norvegicus), dog (Canis familiaris), pig (Sus scrofa) and horse (Equus caballus). |
| Immunogen UniProt ID | Q16678 |
| Positive Control | IHC: formalin-fixed, paraffin-embedded ovarian cancer sections; Western Blot: rhCYP1B1 from lymphoblastoid cells (Gentest); 10mcg microsomal protein / lane |
| Subclass | IgG1 kappa |
| Myeloma Used | P3X63Ag8.653 |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest The majority of breast cancers showed positive immunoreactivity for CYP1B1, and in each case CYP1B1 was specifically localised to tumour cells. Not reactive with CYP1A1. IHC antigen retrieval: microwave 20 min @ 800W in 10mM citrate buffer, pH 6.0. Concentration 1mg/ml as standard |
| Research Area | Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Metabolism, Neurobiology |
References: 10 entries
Carnell et al. 2004. Int J Radiat Oncol Biol Phys. 58(2):500-9. PMID: 14751521.
Target validation of cytochrome P450 CYP1B1 in prostate carcinoma with protein expression in associated hyperplastic and premalignant tissue.
Europe PMC ID: 14751521
Maecker et al. 2003. Blood. 102(9):3287-94. PMID: 12869499.
The shared tumor-associated antigen cytochrome P450 1B1 is recognized by specific cytotoxic T cells.
Europe PMC ID: 12869499
McFadyen et al. 2001. Br J Cancer. 85(2):242-6. PMID: 11461084.
IHC
Cytochrome P450 CYP1B1 over-expression in primary and metastatic ovarian cancer.
Europe PMC ID: 11461084
Murray et al. 2001. Annu Rev Pharmacol Toxicol. 41:297-316. PMID: 11264459.
IHC
Regulation, function, and tissue-specific expression of cytochrome P450 CYP1B1.
Europe PMC ID: 11264459
McFadyen et al. 1999. J Histochem Cytochem. 47(11):1457-64. PMID: 10544218.
IHC
Immunohistochemical localization of cytochrome P450 CYP1B1 in breast cancer with monoclonal antibodies specific for CYP1B1.
Europe PMC ID: 10544218
Add a reference
References: 10 entries
Carnell et al. 2004. Int J Radiat Oncol Biol Phys. 58(2):500-9. PMID: 14751521.
Target validation of cytochrome P450 CYP1B1 in prostate carcinoma with protein expression in associated hyperplastic and premalignant tissue.
Maecker et al. 2003. Blood. 102(9):3287-94. PMID: 12869499.
The shared tumor-associated antigen cytochrome P450 1B1 is recognized by specific cytotoxic T cells.
McFadyen et al. 2001. Br J Cancer. 85(2):242-6. PMID: 11461084.
IHC
Cytochrome P450 CYP1B1 over-expression in primary and metastatic ovarian cancer.
Murray et al. 2001. Annu Rev Pharmacol Toxicol. 41:297-316. PMID: 11264459.
IHC
Regulation, function, and tissue-specific expression of cytochrome P450 CYP1B1.
McFadyen et al. 1999. J Histochem Cytochem. 47(11):1457-64. PMID: 10544218.
IHC
Immunohistochemical localization of cytochrome P450 CYP1B1 in breast cancer with monoclonal antibodies specific for CYP1B1.
Add a reference
