Anti-Protein Kinase C α [133]
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- References (13)
- Inventor Info
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| Catalogue Number | 153646 |
| Applications | IHC WB |
| Antigen/Gene or Protein Targets | Protein Kinase C α |
| Synonyms |
Protein kinase C alpha, PKC-A, PKC-alpha, PRKCA, PKCA, PRKACA |
| Reactivity | Human and Mammals |
| Relevance |
Monoclonal antibody which specifically detects the α isoform of PKC. Background and Research Application The PKC family consists of fifteen isozymes in humans. Protein Kinase C alpha (PKCα) is involved in the regulation of cell proliferation during cell cycle progression. PKC-α is a 672 amino acid enzyme containing a single AGC-kinase C-terminal domain, a C2 domain, 2 phorbol-ester/DAGtype zinc fingers and a single protein kinase domain. Anti-PKC (133) recognizes the α isoform of PKC and binds to a sequence at the C terminus of PKCα to detect its expression in vitro. |
| Host | Mouse |
| Immunogen | Recognizes sequence PQFVHPILQSAV at the C terminus at PKCα |
| Immunogen UniProt ID | P17252 |
| Positive Control | Retina |
| Subclass | IgG2a |
| Molecular Weight (kDa) | 75 |
| Myeloma Used | Sp2/0-Ag14 |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest This antibody recognises the sequence PQFVHPILQSAV at the C terminus of PKCα. Suggested dilutions for use Western Blot 1:10-1:500 Immunohistochemistry 1:10 Research Areas: Cancer; Neurobiology; Pathology; Cell Biology Concentration 1mg/ml as standard |
| Research Area | Cancer, Neurobiology |
References: 13 entries
De Silva et al. 2017. Proc Natl Acad Sci U S A. 114(42):11211-11216. PMID: 28973921.
Wang et al. 2017. Neuron. 93(6):1359-1374.e6. PMID: 28262416.
Kim et al. 2016. Mol Neurodegener. 11:30. PMID: 27098079.
Sarria et al. 2016. J Neurosci. 36(10):2915-25. PMID: 26961947.
Sarria et al. 2015. Elife. 4:e06358. PMID: 25879270.
Donovan et al. 2012. Mol Pharmacol. 82(3):428-37. PMID: 22653970.
Long QT2 mutation on the Kv11.1 ion channel inhibits current activity by ablating a protein kinase Cα consensus site.
Europe PMC ID: 22653970
Cao et al. 2012. Proc Natl Acad Sci U S A. 109(20):7905-10. PMID: 22547806.
Regulators of G protein signaling RGS7 and RGS11 determine the onset of the light response in ON bipolar neurons.
Europe PMC ID: 22547806
Campo et al. 2010. Biochim Biophys Acta. 1802(11):1118-30. PMID: 20713153.
High-molecular weight hyaluronan reduced renal PKC activation in genetically diabetic mice.
Europe PMC ID: 20713153
Huang et al. 2009. Vis Neurosci. 26(5-6):443-52. PMID: 20003598.
Cell type-specific and light-dependent expression of Rab1 and Rab6 GTPases in mammalian retinas.
Europe PMC ID: 20003598
Add a reference
References: 13 entries
De Silva et al. 2017. Proc Natl Acad Sci U S A. 114(42):11211-11216. PMID: 28973921.
Wang et al. 2017. Neuron. 93(6):1359-1374.e6. PMID: 28262416.
Kim et al. 2016. Mol Neurodegener. 11:30. PMID: 27098079.
Sarria et al. 2016. J Neurosci. 36(10):2915-25. PMID: 26961947.
Sarria et al. 2015. Elife. 4:e06358. PMID: 25879270.
Donovan et al. 2012. Mol Pharmacol. 82(3):428-37. PMID: 22653970.
Long QT2 mutation on the Kv11.1 ion channel inhibits current activity by ablating a protein kinase Cα consensus site.
Cao et al. 2012. Proc Natl Acad Sci U S A. 109(20):7905-10. PMID: 22547806.
Regulators of G protein signaling RGS7 and RGS11 determine the onset of the light response in ON bipolar neurons.
Campo et al. 2010. Biochim Biophys Acta. 1802(11):1118-30. PMID: 20713153.
High-molecular weight hyaluronan reduced renal PKC activation in genetically diabetic mice.
Huang et al. 2009. Vis Neurosci. 26(5-6):443-52. PMID: 20003598.
Cell type-specific and light-dependent expression of Rab1 and Rab6 GTPases in mammalian retinas.
Add a reference