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Monoclonal Antibodies

Anti-Deleted in azoospermia-like [DAZL3/11A]

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Image thumbnail for Anti-Deleted in azoospermia-like [DAZL3/11A]
Image thumbnail for Anti-Deleted in azoospermia-like [DAZL3/11A]
Image thumbnail for Anti-Deleted in azoospermia-like [DAZL3/11A]
Image thumbnail for Anti-Deleted in azoospermia-like [DAZL3/11A]
Image thumbnail for Anti-Deleted in azoospermia-like [DAZL3/11A]
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Clone 3/11A used to detect DAZL expression in human ovary cells by Immunofluorescence. (B) Triple fluorescent immunohistochemistry for GDF9 (green), DAZL (blue) and BOLL (red) in 20 week human fetal ovary with DAPI as counterstain (grey). Split channel and merged images in (A) and (B) are shown as are merged images of non-immune serum negative control (NEG). Scale bars are 20μm. (C) Nuclear diameters of DAZL, BOLL and GDF9 stained germ cells indicates that GDF9 positive cells are significantly larger (p<0.001) than DAZL but not BOLL expressing cells (bars indicate mean ± sem). (D) Higher magnification merged image of GDF9/DAZL/BOLL immunohistochemistry showing one large primordial follicle is positive for both GDF9 and DAZL but other follicles are positive only for DAZL. Source: Bayne et al. 2015. PLoS One. 10(3):e0119819. PMID: 25790371.
Clone 3/11A used to detect DAZL expression in human ovary cells by Immunofluorescence. DAZL positive germ cells were rare in the 1st trimester (e, ovary 61 d; g, testis 64 d) but groups of cells ('nests', labelled N) with cytoplasmic staining were present in the 2nd trimester ovaries. Source: Anderson et al. 2007. BMC Dev Biol. 7:136. PMID: 18088417.
Clone 3/11A used to detect DAZL expression in cynomolgus monkey testes by Immunofluorescence on paraffin tissue sections. (D–Q) Expression of VASA, DAZL, and SCP1 proteins during spermatogenesis in cynomolgus monkeys. Source: Yamauchi et al. 2009. PLoS One. 4(4):e5338. PMID: 19399191.
Clone 3/11A used to detect DAZL expression in ovarion tissue by Immunofluorescence. Abnormal germ cell proliferation was noted in GR-deficient embryos after sex determination. Source: Chen et al. 2013. BMC Biol. 11:22. PMID: 23497137.
Clone 3/11A used to detect DAZL expression in rat testis by IHC-P. Immunohistochemistry showing localisation of germ cells and 5mC during mid to late gestation. In order to clearly show the localisation of germ cells at all stages of development... using DAZL as a cytoplasmic marker. Source: Rose et al. 2014. Epigenetics Chromatin. 7:19. PMID: 25225576.
Clone 3/11A used to detect DAZL in ovarian and testicular tissue by Western Blot. Western analysis of 1st and 2nd trimester ovaries and testes. In both ovarian (A) and testicular (B) samples VASA (76 Kd) was not detectable in the 1st trimester samples but was present in those from the 2nd trimester. DAZL (33 Kd) was low/undetectable in 1st trimester ovaries (C) whereas it was detectable in ovarian samples from 2nd trimester and testicular samples from both 1st and 2nd trimester (D). OCT4 (42 Kd) was present in both ovaries (E) and testes (F) during both the 1st and 2nd trimester. The loading control in all cases was β-tubulin (51 Kd). Source: Anderson et al. 2007. BMC Dev Biol. 7:136. PMID: 18088417.
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Catalogue Number 153645
Applications IHC IF WB
Antigen/Gene or Protein Targets Deleted in azoospermia-like
Synonyms Deleted in azoospermia-like, DAZL, DAZ homolog, DAZ-like autosomal, Deleted in azoospermia-like 1, SPGY-like-autosomal, DAZH, DAZL1, DAZLA, SPGYLA
Reactivity Human, Mouse, Rat and Cynomolgus monkey
Relevance Deleted in Azoospermia-like (DAZL) protein plays a role in germ cell development. Mutations in this gene are linked to spermatogenic failure and infertility in males. Clone 3/11A recognizes DAZL at its C terminus, and is used in detection of DAZL expression by various methods.
Host Mouse
Immunogen Recognizes DAZL protein C terminus sequence CRVHHFRRSRAMLKSV
Positive Control Testis or Ovary
Subclass IgG1
Molecular Weight (kDa) 33
Myeloma Used Sp2/0-Ag14
Notes Suggested dilutions for use

Western Blot 1:100-1:1000
Immunohistochemistry 1:50-1:500
Immunofluorescence 1:50-1:500

Research Areas: Endocrinology;Fertility;Genetics;Developmental Biology;Stem Cell Biology
Research Area Developmental Biology, Stem Cell Biology

References

There are 10 reference entries for this reagent.

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References: 10 entries

GDF9 is transiently expressed in oocytes before follicle formation in the human fetal ovary and is regulated by a novel NOBOX transcript.

Europe PMC ID: 25790371

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Bayne et al. 2015. PLoS One. 10(3):e0119819. PMID: 25790371.

View  

Dynamic changes in DNA modification states during late gestation male germ line development in the rat.

Europe PMC ID: 25225576

View  

Rose et al. 2014. Epigenetics Chromatin. 7:19. PMID: 25225576.

View  

Chen et al. 2013. BMC Biol. 11:22. PMID: 23497137.

View  

Disruption of genital ridge development causes aberrant primordial germ cell proliferation but does not affect their directional migration.

Europe PMC ID: 23497137

View  

In vitro germ cell differentiation from cynomolgus monkey embryonic stem cells.

Europe PMC ID: 19399191

View  

Yamauchi et al. 2009. PLoS One. 4(4):e5338. PMID: 19399191.

View  

Anderson et al. 2007. BMC Dev Biol. 7:136. PMID: 18088417.

View  

Conserved and divergent patterns of expression of DAZL, VASA and OCT4 in the germ cells of the human fetal ovary and testis.

Europe PMC ID: 18088417

View  

Add a reference

References: 10 entries

GDF9 is transiently expressed in oocytes before follicle formation in the human fetal ovary and is regulated by a novel NOBOX transcript.

View  

Bayne et al. 2015. PLoS One. 10(3):e0119819. PMID: 25790371.

View  

Dynamic changes in DNA modification states during late gestation male germ line development in the rat.

View  

Rose et al. 2014. Epigenetics Chromatin. 7:19. PMID: 25225576.

View  

Chen et al. 2013. BMC Biol. 11:22. PMID: 23497137.

View  

Disruption of genital ridge development causes aberrant primordial germ cell proliferation but does not affect their directional migration.

View  

In vitro germ cell differentiation from cynomolgus monkey embryonic stem cells.

View  

Yamauchi et al. 2009. PLoS One. 4(4):e5338. PMID: 19399191.

View  

Anderson et al. 2007. BMC Dev Biol. 7:136. PMID: 18088417.

View  

Conserved and divergent patterns of expression of DAZL, VASA and OCT4 in the germ cells of the human fetal ovary and testis.

View  

Add a reference

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