Anti-Myelin Basic Protein (region Thr98) [98/P12]
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- References (10)
- Inventor Info
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| Catalogue Number | 153644 |
| Applications | ELISA WB |
| Antigen/Gene or Protein Targets | Myelin Basic Protein (region Thr98) |
| Synonyms |
Myelin basic protein, MBP, 20 kDa microtubule-stabilizing protein, Myelin A1 protein |
| Reactivity | Human |
| Relevance | The phosphorylation of myelin basic protein (MBP) has been shown to decrease the ability of MBP to aggregate lipid vesicles and consequently destabilising the compact structure of myelin, a destruction that has been observed in demyelinating diseases such as Multiple Sclerosis. (Yon, M et al. 1995) Clone 98/P12 is a useful detector of phosphorylated MBP by binding to Thr98 of human MBP in the phosphorylated state. |
| Host | Mouse |
| Immunogen | Synthetic peptide corresponding to human MBP when phosphorylated at threonine 98 |
| Positive Control | Brain tissue |
| Subclass | IgG2a |
| Molecular Weight (kDa) | 13-21 |
| Myeloma Used | Sp2/0-Ag14 |
| Notes |
Suggested dilutions for use Western Blot 1:1000-1:2000 Immunocytochemistry 1:400 ELISA Assay-dependent Research Areas: Neurobiology; Cell Type or Organelle Marker; Immunology |
| Research Area | Cell Type or Organelle Marker, Immunology, Neurobiology |
References: 10 entries
DeBruin et al. 2006. Biochem Cell Biol. 84(6):993-1005. PMID: 17215885.
Partitioning of myelin basic protein into membrane microdomains in a spontaneously demyelinating mouse model for multiple sclerosis.
Europe PMC ID: 17215885
DeBruin et al. 2005. J Neurosci Res. 80(2):211-25. PMID: 15772981.
Developmental partitioning of myelin basic protein into membrane microdomains.
Europe PMC ID: 15772981
Atkins et al. 1999. J Neurochem. 73(3):1090-7. PMID: 10461899.
Regulation of myelin basic protein phosphorylation by mitogen-activated protein kinase during increased action potential firing in the hippocampus.
Europe PMC ID: 10461899
Yon et al. 1996. J Neuroimmunol. 65(1):55-9. PMID: 8642064.
Identification of a mitogen-activated protein kinase site in human myelin basic protein in situ.
Europe PMC ID: 8642064
Yon et al. 1995. J Neuroimmunol. 58(2):121-9. PMID: 7759601.
Preparation of a novel monoclonal antibody specific for myelin basic protein phosphorylated on Thr98.
Europe PMC ID: 7759601
Add a reference
References: 10 entries
DeBruin et al. 2006. Biochem Cell Biol. 84(6):993-1005. PMID: 17215885.
Partitioning of myelin basic protein into membrane microdomains in a spontaneously demyelinating mouse model for multiple sclerosis.
DeBruin et al. 2005. J Neurosci Res. 80(2):211-25. PMID: 15772981.
Developmental partitioning of myelin basic protein into membrane microdomains.
Atkins et al. 1999. J Neurochem. 73(3):1090-7. PMID: 10461899.
Regulation of myelin basic protein phosphorylation by mitogen-activated protein kinase during increased action potential firing in the hippocampus.
Yon et al. 1996. J Neuroimmunol. 65(1):55-9. PMID: 8642064.
Identification of a mitogen-activated protein kinase site in human myelin basic protein in situ.
Yon et al. 1995. J Neuroimmunol. 58(2):121-9. PMID: 7759601.
Preparation of a novel monoclonal antibody specific for myelin basic protein phosphorylated on Thr98.
Add a reference