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Monoclonal Antibodies

Anti-Estrogen Receptor ß5 [PPG5/25]

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Clone PPG5/25 used to detect ERβ5 expression thymic tumors by Immunofluorescence. (A)Overexpression of cytoplasmic ERβ5 was observed in thymic carcinomas and thymomas, compared with the normal thymic tissues. (B) ERβ5 expression was observed in the T1682 and TC1889 cell lines through indirect immunofluorescence. (C) The results revealed that the mean level of ERβ5 gene expression was lower in advanced clinical stages. ERβ5, estrogen receptor β5. Source: Li et al. 2015. Oncol Lett. 10(4):2341-2346. PMID: 26622848.
Clone PPG5/25 used to detect expression of ERβ5 in cancer tissue by IHC-P. Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; main panels show closely adjacent sections from three cancer blocks to allow direct comparisons. Source: Collins et al. 2009. BMC Cancer. 9:330. PMID: 19758455.
Clone PPG5/25 used to detect expression of ERβ5 in cancer tissue by IHC-P. A) Immunostaining for ERβ1 was significantly higher in tumors from females compared to males (**p<0.01); B) and C) Tumor levels of ERβ2 and ERβ5 did not significantly differ between females and males. All results are expressed as the mean ± SEM (n = 6 tumors/group). Representative stained section of tumors from females and males are shown (magnification 40x). IHC, immunohistochemical score. Source: Ciucci et al. 2014. PLoS One. 9(7):e101623. PMID: 25000562.
Clone PPG5/25 used to detect ERβ5 expression in normal human liver by IHC-P. Staining pattern of the ERß isoforms in human liver. In order to confirm the specificity of the ERß antibodies used in the study, we immunostained sections of human healthy liver, showing that ERß1 and ERß2 isoforms are not expressed, while specific nuclear ERß5 immunoreactivity is detected (magnification 20x and 40x). This pattern of ERß isoforms immunoreactivity is consistent with previous descriptions. Source: Ciucci et al. 2014. PLoS One. 9(7):e101623. PMID: 25000562.
Clone PPG5/25 used to detect expression of ERβ5 in colorectal tissue by IHC-P. Examples of ERβ1 (a, d, e), ERβ2 (b, f, g), ERβ5 (c, h) and TFF1 (i) immunohistochemistry performed on normal colorectal epithelium (a–c) and colorectal carcinoma (d–i). Source: Wong et al. 2005. J Pathol. 207(1):53-60. PMID: 15954165.
Clone PPG5/25 used to detect ERβ5 mRNA expression in colorectal carcinoma cells by Western Blot. (A) ERβ1, 2 and 5 mRNA expression (assessed by RT-PCR) and (B) ERβ1 and 2 protein expression (assessed by western blotting) by 10 colorectal carcinoma cell lines. Source: Wong et al. 2005. J Pathol. 207(1):53-60. PMID: 15954165.
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Catalogue Number 153633
Applications IHC IF WB
Antigen/Gene or Protein Targets Estrogen Receptor β 5
Synonyms Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2
Reactivity Human
Relevance Estrogen receptors are commonly over-expressed in cancers. Clone PPG5/25 recognizes Estrogen Receptor Beta (ERβ) 5 and is useful in determining the expression of ERβ5 in cancer cells, mainly via immunohistochemistry.
Host Mouse
Immunogen Tuberculin conjugated peptide that recognizes C terminus of ERβ5 at sequence LLSHVRHARYAP
Positive Control Normal colon or colon carcinoma
Subclass IgG1
Myeloma Used Sp2/0-Ag14
Notes Suggested dilutions for use

Western Blot Assay-dependent
Immunohistochemistry 1:50-1:100
Immunofluorescence 1:500

Research Areas: Endocrinology;Cancer;Immunology;Neurobiology;Hepatology
Research Area Cancer, Immunology, Neurobiology

References

There are 10 reference entries for this reagent.

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References: 10 entries

Zannoni et al. 2016. Histopathology. 68(4):541-8. PMID: 26132417.

View  

Li et al. 2015. Oncol Lett. 10(4):2341-2346. PMID: 26622848.

View  

Sexual dimorphism in medulloblastoma features.

Europe PMC ID: 26132417

View  

Cytoplasm estrogen receptor β5 as an improved prognostic factor in thymoma and thymic carcinoma progression.

Europe PMC ID: 26622848

View  

Gender effect in experimental models of human medulloblastoma: does the estrogen receptor β signaling play a role?

Europe PMC ID: 25000562

View  

Ciucci et al. 2014. PLoS One. 9(7):e101623. PMID: 25000562.

View  

Collins et al. 2009. BMC Cancer. 9:330. PMID: 19758455.

View  

Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha.

Europe PMC ID: 19758455

View  

Wong et al. 2005. J Pathol. 207(1):53-60. PMID: 15954165.

View  

ERbeta isoform expression in colorectal carcinoma: an in vivo and in vitro study of clinicopathological and molecular correlates.

Europe PMC ID: 15954165

View  

Add a reference

References: 10 entries

Zannoni et al. 2016. Histopathology. 68(4):541-8. PMID: 26132417.

View  

Li et al. 2015. Oncol Lett. 10(4):2341-2346. PMID: 26622848.

View  

Sexual dimorphism in medulloblastoma features.

View  

Cytoplasm estrogen receptor β5 as an improved prognostic factor in thymoma and thymic carcinoma progression.

View  

Gender effect in experimental models of human medulloblastoma: does the estrogen receptor β signaling play a role?

View  

Ciucci et al. 2014. PLoS One. 9(7):e101623. PMID: 25000562.

View  

Collins et al. 2009. BMC Cancer. 9:330. PMID: 19758455.

View  

Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha.

View  

Wong et al. 2005. J Pathol. 207(1):53-60. PMID: 15954165.

View  

ERbeta isoform expression in colorectal carcinoma: an in vivo and in vitro study of clinicopathological and molecular correlates.

View  

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