Anti-Estrogen Receptor ß2 [57/3]

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Clone 57/3 used to detect expression of ERβ2 in ovarian cancer cells by Immunofluorescence. A) Representative pictures for ERα, ERβ1, ERβ2, ERβ5, PR, and AR immunostaining in LGSOC patients (magnification 20 ×) and bar chart showing levels of hormone receptor expression (mean ± SEM, n = 25). Results are expressed as IRS (Immunoreactive receptor score). B) Representative pictures showing exclusive nuclear and nuclear/cytoplasmic ERβ2 expression in primary tumor and matched peritoneal metastasis. Source: Buttarelli et al. 2017. Gynecol Oncol. 145(2):352-360. PMID: 28228232.

Clone 57/3 used to detect expression of ERβ2 in cancer tissue by IHC-P. Immunoexpression of ERs in endometrial cancers. Tissues were classified as well (A-D), moderately (E-H) or poorly (J-M) differentiated; ...immunoexpression of ERβ1, 2, 5 was readily detected (K. L, M). Inserts in panels K, L, and M show negative controls for ERβ1, ERβ2 and ERβ5 antibodies respectively generated using primary antibodies pre-absorbed with specific peptides used for immunisation. Asterisks (*) label the stromal compartment that was well defined in the well differentiated cancers. Source: Collins et al. 2009. BMC Cancer. 9:330. PMID: 19758455.

Clone 57/3 used to detect expression of ERβ2 in cancer tissue by IHC-P. A) Immunostaining for ERβ1 was significantly higher in tumors from females compared to males (**p<0.01); B) and C) Tumor levels of ERβ2 and ERβ5 did not significantly differ between females and males. All results are expressed as the mean ± SEM (n = 6 tumors/group). Representative stained section of tumors from females and males are shown (magnification 40x). IHC, immunohistochemical score. Source: Ciucci et al. 2014. PLoS One. 9(7):e101623. PMID: 25000562.

Clone 57/3 used to detect ERβ2 expression in normal human liver by IHC-P. Staining pattern of the ERß isoforms in human liver. In order to confirm the specificity of the ERß antibodies used in the study, we immunostained sections of human healthy liver, showing that ERß1 and ERß2 isoforms are not expressed, while specific nuclear ERß5 immunoreactivity is detected (magnification 20x and 40x). This pattern of ERß isoforms immunoreactivity is consistent with previous descriptions. Source: Ciucci et al. 2014. PLoS One. 9(7):e101623. PMID: 25000562.

Clone 57/3 used to detect expression of ERβ2 in colorectal tissue by IHC-P. Examples of ERβ1 (a, d, e), ERβ2 (b, f, g), ERβ5 (c, h) and TFF1 (i) immunohistochemistry performed on normal colorectal epithelium (a–c) and colorectal carcinoma (d–i). Source: Wong et al. 2005. J Pathol. 207(1):53-60. PMID: 15954165.

Clone 57/3 used to detect expression of ERβ2 in Leydig cell tumors by IHC-P. Morphology and immunolocalization of aromatase and estrogen receptors in Leydig cell tumors (A and B) and control testis (C). A–C: Hematoxylin–eosin staining. A1–C1: Aromatase immunolocalization. A2–C2: ERα immunoreactivity. A3–C3: ERβ1 immunodetection. A4–C4: ERβ2 immunolocalization. L, Leydig cells; T, seminiferous tubule. Source: Carpino et al. 2007. Eur J Endocrinol. 157(2):239-44. PMID: 17656605.

Clone 57/3 used to detect expression of ERβ2 in colorectal tissue by Western Blot. (A) ERβ1, 2 and 5 mRNA expression (assessed by RT-PCR) and (B) ERβ1 and 2 protein expression (assessed by western blotting) by 10 colorectal carcinoma cell lines. Source: Wong et al. 2005. J Pathol. 207(1):53-60. PMID: 15954165.

Clone 57/3 used to detect expression of ERβ2 in Leydig cell tumors by Western Blot. Immunoblots of aromatase and estrogen receptors from protein extracts of Leydig cell tumors and control tissues. P450arom: rat ovary (C+), negative control (C−), Leydig cell tumors (lanes 1 and 2). ERα: rat ovary (C+), negative control (C−), Leydig cell tumors (lanes 1 and 2). ERβ1: control human testis (C+), negative control (C−), Leydig cell tumors (lanes 1 and 2). ERβ2: control human testis (C+), negative control (C−), Leydig cell tumors (lanes 1 and 2). β-Actin serves as a loading control. Source: Carpino et al. 2007. Eur J Endocrinol. 157(2):239-44. PMID: 17656605.

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Catalogue Number	153632
Applications	IHC IF WB
Antigen/Gene or Protein Targets	Estrogen Receptor β 2
Synonyms	Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2
Reactivity	Human
Relevance	Estrogen receptors are commonly over-expressed in cancers. Clone 57/3 recognizes Estrogen Receptor Beta (ERβ) 2 and is useful in determining the expression of ERβ2 in cancer cells via various analysis methods.
Host	Mouse
Immunogen	Recognizes ERβ2 isomer at sequence CGMKMETLLPEATMEQ
Positive Control	Ovary
Subclass	IgG1
Molecular Weight (kDa)	53
Myeloma Used	Sp2/0-Ag14
Strain	Balb/c
Notes	Suggested dilutions for use Western Blot 1:500 Immunohistochemistry 1:50-1:500 Immunofluorescence 1:100 Research Areas: Endocrinology;Cancer;Fertility;Immunology;Hepatology
Research Area	Cancer, Immunology

References

There are 20 reference entries for this reagent.

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References: 20 entries

Buttarelli et al. 2017. Gynecol Oncol. 145(2):352-360. PMID: 28228232.

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Hormone receptor expression profile of low-grade serous ovarian cancers.

Europe PMC ID: 28228232

View

Zannoni et al. 2016. Histopathology. 68(4):541-8. PMID: 26132417.

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Sexual dimorphism in medulloblastoma features.

Europe PMC ID: 26132417

View

Gender effect in experimental models of human medulloblastoma: does the estrogen receptor β signaling play a role?

Europe PMC ID: 25000562

View

Ciucci et al. 2014. PLoS One. 9(7):e101623. PMID: 25000562.

View

Collins et al. 2009. BMC Cancer. 9:330. PMID: 19758455.

View

Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha.

Europe PMC ID: 19758455

View

Carpino et al. 2007. Eur J Endocrinol. 157(2):239-44. PMID: 17656605.

View

Detection of aromatase and estrogen receptors (ERalpha, ERbeta1, ERbeta2) in human Leydig cell tumor.

Europe PMC ID: 17656605

View

Skliris et al. 2006. Br J Cancer. 95(5):616-26. PMID: 16880783.

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Expression of oestrogen receptor-beta in oestrogen receptor-alpha negative human breast tumours.

Europe PMC ID: 16880783

View

Wong et al. 2005. J Pathol. 207(1):53-60. PMID: 15954165.

View

ERbeta isoform expression in colorectal carcinoma: an in vivo and in vitro study of clinicopathological and molecular correlates.

Europe PMC ID: 15954165

View

Sierens et al. 2004. J Mol Endocrinol. 32(3):703-18. PMID: 15171710.

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Cloning of oestrogen receptor beta from Old and New World primates: identification of splice variants and functional analysis.

Europe PMC ID: 15171710

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Critchley et al. 2002. J Clin Endocrinol Metab. 87(11):5265-73. PMID: 12414901.

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Wild-type estrogen receptor (ERbeta1) and the splice variant (ERbetacx/beta2) are both expressed within the human endometrium throughout the normal menstrual cycle.

Europe PMC ID: 12414901

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Ogawa et al. 1998. Nucleic Acids Res. 26(15):3505-12. PMID: 9671811.

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Molecular cloning and characterization of human estrogen receptor betacx: a potential inhibitor ofestrogen action in human.

Europe PMC ID: 9671811

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References: 20 entries

Buttarelli et al. 2017. Gynecol Oncol. 145(2):352-360. PMID: 28228232.

View

Hormone receptor expression profile of low-grade serous ovarian cancers.

View

Zannoni et al. 2016. Histopathology. 68(4):541-8. PMID: 26132417.

View

Sexual dimorphism in medulloblastoma features.

View

Gender effect in experimental models of human medulloblastoma: does the estrogen receptor β signaling play a role?

View

Ciucci et al. 2014. PLoS One. 9(7):e101623. PMID: 25000562.

View

Collins et al. 2009. BMC Cancer. 9:330. PMID: 19758455.

View

Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha.

View

Carpino et al. 2007. Eur J Endocrinol. 157(2):239-44. PMID: 17656605.

View

Detection of aromatase and estrogen receptors (ERalpha, ERbeta1, ERbeta2) in human Leydig cell tumor.

View

Skliris et al. 2006. Br J Cancer. 95(5):616-26. PMID: 16880783.

View

Expression of oestrogen receptor-beta in oestrogen receptor-alpha negative human breast tumours.

View

Wong et al. 2005. J Pathol. 207(1):53-60. PMID: 15954165.

View

ERbeta isoform expression in colorectal carcinoma: an in vivo and in vitro study of clinicopathological and molecular correlates.

View

Sierens et al. 2004. J Mol Endocrinol. 32(3):703-18. PMID: 15171710.

View

Cloning of oestrogen receptor beta from Old and New World primates: identification of splice variants and functional analysis.

View

Critchley et al. 2002. J Clin Endocrinol Metab. 87(11):5265-73. PMID: 12414901.

View

Wild-type estrogen receptor (ERbeta1) and the splice variant (ERbetacx/beta2) are both expressed within the human endometrium throughout the normal menstrual cycle.

View

Ogawa et al. 1998. Nucleic Acids Res. 26(15):3505-12. PMID: 9671811.

View

Molecular cloning and characterization of human estrogen receptor betacx: a potential inhibitor ofestrogen action in human.

View

Add a reference

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Anti-Estrogen Receptor ß2 [57/3]

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References: 20 entries

References: 20 entries

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