- Datasheet
- References (6)
- Inventor Info
Info
| Catalogue Number | 153626 |
| Synonyms | Ventral Otocyst-Neuroblast cell line number 33 |
| Host | Mouse |
| Model | Immortalised Line |
| Relevance |
Derived from migrating neuroblasts in the ventral region of the otocyst at embryonic day E10.5 (plug in mouse designated E0.5 and birth at E18-19). At this stage the neuroblasts have not exited the cell cycle and have not differentiated. VOT-N33 has been characterised extensively by timed expression under differentiating conditions in vitro of a combination of gene and protein markers for inner ear neurons. It has also been screened with Affymetrix mouse Micro-arrays. It forms exclusively bipolar neuronal phenotypes under differentiating conditions in vitro in the presence of FGFs and also following transplantation to the cochlear nerve in vivo. The cells are also available with a stably incorporated EGFP reporter that has been tested both in vitro and in vivo. Neuroblast cell line suitable for studies on: Inner ear development Gene expression and function of inner ear-specific genes In vitro screening for gene activation and promoter analysis Ototoxicity (prescribed drugs and agents that ameliorate their affects) Function of inherited deafness mutations Functional analysis of ion channels, receptors and signalling pathways in vitro |
| Conditional | Yes |
| Conditional Description | Cells derived from C57 Bl6 mice carrying a stable insertion of the conditional immortalising gene H-2Kb-tsA58, which describes a temperature-sensitive variant of the SV40 immortalising gene that encodes the large tumour antigen under the control of the γ–interferon-sensitive MHC Class 1 promoter. The transgenic mouse is called the Immortomouse™ (Jat et al 1991 Proc. Nat. Acad. Sci. USA 88, 5096-5100) |
| Research Area | Developmental Biology, Drug Discovery & Development, Gene Expression |
| Recommended Growing Conditions | MEM with 10% FCS, 50Units/ml y-IFN, L-glutamine, 33°C |
References: 6 entries
Sekiya et al. 2007. Eur J Neurosci. 25(8):2307-18. PMID: 17445229.
Transplantation of conditionally immortal auditory neuroblasts to the auditory nerve.
Europe PMC ID: 17445229
Lawoko-Kerali et al. 2004. Dev Dyn. 231(4):801-14. PMID: 15499550.
Ventral otic cell lines as developmental models of auditory epithelial and neural precursors.
Europe PMC ID: 15499550
Kaufman et al. 1967. Calif Med. 107(1):48-51. PMID: 6045487.
Renal carcinoma as an accidental finding on needle biopsy.
Europe PMC ID: 6045487
Add a reference
References: 6 entries
Sekiya et al. 2007. Eur J Neurosci. 25(8):2307-18. PMID: 17445229.
Transplantation of conditionally immortal auditory neuroblasts to the auditory nerve.
Lawoko-Kerali et al. 2004. Dev Dyn. 231(4):801-14. PMID: 15499550.
Ventral otic cell lines as developmental models of auditory epithelial and neural precursors.
Kaufman et al. 1967. Calif Med. 107(1):48-51. PMID: 6045487.
Renal carcinoma as an accidental finding on needle biopsy.
Add a reference
Inventor Information
Inventors
|
Matthew C Holley |
