Cancer Research Technology
Log in Register
Menu

US/VOT-N33 Cell Line

Info

Catalogue Number 153626
Synonyms Ventral Otocyst-Neuroblast cell line number 33
Host Mouse
Model Immortalised Line
Relevance Derived from migrating neuroblasts in the ventral region of the otocyst at embryonic day E10.5 (plug in mouse designated E0.5 and birth at E18-19). At this stage the neuroblasts have not exited the cell cycle and have not differentiated. VOT-N33 has been characterised extensively by timed expression under differentiating conditions in vitro of a combination of gene and protein markers for inner ear neurons. It has also been screened with Affymetrix mouse Micro-arrays. It forms exclusively bipolar neuronal phenotypes under differentiating conditions in vitro in the presence of FGFs and also following transplantation to the cochlear nerve in vivo. The cells are also available with a stably incorporated EGFP reporter that has been tested both in vitro and in vivo.

Neuroblast cell line suitable for studies on:
Inner ear development
Gene expression and function of inner ear-specific genes
In vitro screening for gene activation and promoter analysis
Ototoxicity (prescribed drugs and agents that ameliorate their affects)
Function of inherited deafness mutations
Functional analysis of ion channels, receptors and signalling pathways in vitro
Conditional Yes
Conditional Description Cells derived from C57 Bl6 mice carrying a stable insertion of the conditional immortalising gene H-2Kb-tsA58, which describes a temperature-sensitive variant of the SV40 immortalising gene that encodes the large tumour antigen under the control of the γ–interferon-sensitive MHC Class 1 promoter. The transgenic mouse is called the Immortomouse™ (Jat et al 1991 Proc. Nat. Acad. Sci. USA 88, 5096-5100)
Research Area Developmental Biology, Drug Discovery & Development, Gene Expression
Recommended Growing Conditions MEM with 10% FCS, 50Units/ml y-IFN, L-glutamine, 33°C

References

There are 6 reference entries for this reagent.

View All References

References: 6 entries

Sekiya et al. 2007. Eur J Neurosci. 25(8):2307-18. PMID: 17445229.

Transplantation of conditionally immortal auditory neuroblasts to the auditory nerve.

Europe PMC ID: 17445229

Lawoko-Kerali et al. 2004. Dev Dyn. 231(4):801-14. PMID: 15499550.

Ventral otic cell lines as developmental models of auditory epithelial and neural precursors.

Europe PMC ID: 15499550

Kaufman et al. 1967. Calif Med. 107(1):48-51. PMID: 6045487.

Renal carcinoma as an accidental finding on needle biopsy.

Europe PMC ID: 6045487


Add a reference

References: 6 entries

Sekiya et al. 2007. Eur J Neurosci. 25(8):2307-18. PMID: 17445229.

Transplantation of conditionally immortal auditory neuroblasts to the auditory nerve.

Lawoko-Kerali et al. 2004. Dev Dyn. 231(4):801-14. PMID: 15499550.

Ventral otic cell lines as developmental models of auditory epithelial and neural precursors.

Kaufman et al. 1967. Calif Med. 107(1):48-51. PMID: 6045487.

Renal carcinoma as an accidental finding on needle biopsy.


Add a reference

Inventor Information

No inventors are currently linked to this reagent.

Add an inventor