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UB-UE1 Cell Line

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Catalogue Number 153624
Synonyms Utricular Epithelium cell line number 1
Host Mouse
Model Immortalised Line
Relevance Derived from vestibular epithelium (utricular macula) using thermolysin treatment and dissection to ensure origin from sensory epithelial supporting cells at post-natal day P2. At this stage the supporting cells are normally post-mitotic but retain the ability to differentiate as sensory hair cells and or supporting cells. UB/UE-1 was characterised by timed expression under differentiating conditions in vitro of a combination of gene and protein markers for epithelial cells and for inner ear sensory cells. These include Cytokeratin, Vimentin, Myosin VIIa, Myosin VI, Brn3c, α9AChR and a combination of functional ion channels (see references). Screened with Affymetrix mouse Micro-arrays. The cells differentiate neonatal stage hair cell and supporting cell phenotypes under differentiating conditions in vitro.

Epithelial cell line suitable for studies on:
Inner ear development
Gene expression and function of inner ear-specific genes
In vitro screening for gene activation and promoter analysis
Ototoxicity (prescribed drugs and agents that ameliorate their affects)
Function of inherited deafness mutations
Functional analysis of ion channels, receptors and signalling pathways in vitro
Conditional Yes
Conditional Description Cells derived from C57 Bl6 mice carrying a stable insertion of the conditional immortalising gene H-2Kb-tsA58, which describes a temperature-sensitive variant of the SV40 immortalising gene that encodes the large tumour antigen under the control of the γ–interferon-sensitive MHC Class 1 promoter. The transgenic mouse is called the Immortomouse™ (Jat et al 1991 Proc. Nat. Acad. Sci. USA 88, 5096-5100).
Research Area Developmental Biology, Drug Discovery & Development, Gene Expression
Recommended Growing Conditions MEM with 10% FCS, 50Units/ml y-IFN, L-glutamine, 33°C

References

There are 4 reference entries for this reagent.

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References: 4 entries

Hackett et al. 2002. Exp Cell Res. 278(1):19-30. PMID: 12126954.

E-cadherin and the differentiation of mammalian vestibular hair cells.

Europe PMC ID: 12126954

Lawlor et al. 1999. J Neurosci. 19(21):9445-58. PMID: 10531448.

Differentiation of mammalian vestibular hair cells from conditionally immortal, postnatal supporting cells.

Europe PMC ID: 10531448


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References: 4 entries

Hackett et al. 2002. Exp Cell Res. 278(1):19-30. PMID: 12126954.

E-cadherin and the differentiation of mammalian vestibular hair cells.

Lawlor et al. 1999. J Neurosci. 19(21):9445-58. PMID: 10531448.

Differentiation of mammalian vestibular hair cells from conditionally immortal, postnatal supporting cells.


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