UB-OC1 Cell Line
Invented by Matthew C Holley
Invented at University Of Bristol , University Of Sheffield
- Datasheet
- References (10)
- Inventor Info
Info
| Catalogue Number | 153622 |
| Parental Line | E13 embryonic Immortomouse organs of Corti |
| Synonyms | Organ of Corti cell line number 1 |
| Host | Mouse |
| Model | Immortalised Line |
| Relevance |
Derived from E13 embryonic Immortomouse organs of Corti. The immortalizing gene was activated in organotypic cultures of auditory sensory epithelia at E13, the thirteenth day of embryonic development, before the hair cells had started to differentiate after their last mitoses. The cell line expresses characteristic hair cell markers: the transcription factor Brn3.1, the a9 subunit of the acetylcholine receptor, the stereociliary protein fimbrin and the myosins VI and VIIA. These hair cell precursors permit functional studies of cochlear genes and provide the means to explore therapeutic methods of stimulating auditory hair cell regeneration, substantially facilitate auditory research, drug development, and the isolation of critical molecules involved in hair cell biology. |
| Conditional | Yes |
| Conditional Description | Cells derived from C57 Bl6 mice carrying a stable insertion of the conditional immortalising gene H-2Kb-tsA58, which describes a temperature-sensitive variant of the SV40 immortalising gene that encodes the large tumour antigen under the control of the γ–interferon-sensitive MHC Class 1 promoter. The transgenic mouse is called the Immortomouse™ (Jat et al 1991 Proc. Nat. Acad. Sci. USA 88, 5096-5100). |
| Research Area | Developmental Biology, Drug Discovery & Development, Gene Expression |
| Recommended Growing Conditions | MEM with Earle's salts and 10% FCS, 50Units/ml y-IFN, L-glutamine, 33°C |
References: 10 entries
Clough et al. 2004. Biochem Biophys Res Commun. 324(1):372-81. PMID: 15465029.
Brn-3c (POU4F3) regulates BDNF and NT-3 promoter activity.
Europe PMC ID: 15465029
Rivolta et al. 2002. Genome Res. 12(7):1091-9. PMID: 12097346.
Transcript profiling of functionally related groups of genes during conditional differentiation of a mammalian cochlear hair cell line.
Europe PMC ID: 12097346
Jagger et al. 2000. J Physiol. 527 Pt 1:49-54. PMID: 11011664.
Calcium signalling mediated by the 9 acetylcholine receptor in a cochlear cell line from the immortomouse.
Europe PMC ID: 11011664
Jagger et al. 1999. Pflugers Arch. 438(1):8-14. PMID: 10370081.
Ionic currents expressed in a cell line derived from the organ of Corti of the Immortomouse.
Europe PMC ID: 10370081
Rivolta et al. 1998. Proc Biol Sci. 265(1406):1595-603. PMID: 9753783.
Auditory hair cell precursors immortalized from the mammalian inner ear.
Europe PMC ID: 9753783
Add a reference
References: 10 entries
Clough et al. 2004. Biochem Biophys Res Commun. 324(1):372-81. PMID: 15465029.
Brn-3c (POU4F3) regulates BDNF and NT-3 promoter activity.
Rivolta et al. 2002. Genome Res. 12(7):1091-9. PMID: 12097346.
Transcript profiling of functionally related groups of genes during conditional differentiation of a mammalian cochlear hair cell line.
Jagger et al. 2000. J Physiol. 527 Pt 1:49-54. PMID: 11011664.
Calcium signalling mediated by the 9 acetylcholine receptor in a cochlear cell line from the immortomouse.
Jagger et al. 1999. Pflugers Arch. 438(1):8-14. PMID: 10370081.
Ionic currents expressed in a cell line derived from the organ of Corti of the Immortomouse.
Rivolta et al. 1998. Proc Biol Sci. 265(1406):1595-603. PMID: 9753783.
Auditory hair cell precursors immortalized from the mammalian inner ear.
Add a reference
Inventor Information
Inventors
|
Matthew C Holley |
