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YIp211-P30-His-pol30(K127R)-3' Plasmid

Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories

Info

Catalogue Number 153616
Backbone Size (bp) 3797
Bacterial Resistance Ampicillin
Selectable Markers URA3
Vector Type YIplac211
Synonyms PCNA, Proliferating cell nuclear antigen
Antigen/Gene or Protein Targets POL30 (Saccharomyces cerevisiae)
Relevance Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 marker. Proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.
Research Area Cell Cycle, DNA Damage and Repair, Epitope Tags

References

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References: 1 entry

Detection of PCNA modifications in Saccharomyces cerevisiae. Davies AA, Ulrich HD Methods Mol Biol. 2012;920:543-67. doi: 10.1007/978-1-61779-998-3_36.


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References: 1 entry

Detection of PCNA modifications in Saccharomyces cerevisiae. Davies AA, Ulrich HD Methods Mol Biol. 2012;920:543-67. doi: 10.1007/978-1-61779-998-3_36.


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Inventor Information