YIp128-P30-His-pol30(K127/164R)
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
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Info
Catalogue Number | 153614 |
Backbone Size (bp) | 4293 |
Bacterial Resistance | Ampicillin |
Selectable Markers | LEU2 |
Vector Type | YIplac128 |
Synonyms | PCNA, Proliferating cell nuclear antigen |
Antigen/Gene or Protein Targets | POL30 (Saccharomyces cerevisiae) |
Relevance | Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; Leu2 marker. Proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair. |
Research Area | Cell Cycle, DNA Damage and Repair, Epitope Tags |
References: 1 entry
Detection of PCNA modifications in Saccharomyces cerevisiae. Davies AA, Ulrich HD Methods Mol Biol. 2012;920:543-67. doi: 10.1007/978-1-61779-998-3_36.
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References: 1 entry
Detection of PCNA modifications in Saccharomyces cerevisiae. Davies AA, Ulrich HD Methods Mol Biol. 2012;920:543-67. doi: 10.1007/978-1-61779-998-3_36.
Add a reference