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YIp128-P30-His-pol30(K127R) Plasmid

Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories

Info

Catalogue Number 153612
Backbone Size (bp) 4293
Bacterial Resistance Ampicillin
Selectable Markers LEU2
Vector Type YIplac128
Synonyms PCNA, Proliferating cell nuclear antigen
Antigen/Gene or Protein Targets POL30 (Saccharomyces cerevisiae)
Relevance Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; Leu2 marker, Proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.
Research Area Cell Cycle, DNA Damage and Repair, Epitope Tags

References

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References: 1 entry

Detection of PCNA modifications in Saccharomyces cerevisiae. Davies AA, Ulrich HD Methods Mol Biol. 2012;920:543-67. doi: 10.1007/978-1-61779-998-3_36.


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References: 1 entry

Detection of PCNA modifications in Saccharomyces cerevisiae. Davies AA, Ulrich HD Methods Mol Biol. 2012;920:543-67. doi: 10.1007/978-1-61779-998-3_36.


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Inventor Information