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pRS423-CUP1-His-Ubi Plasmid

Info

Catalogue Number 153609
Backbone Size (bp) 5797
Bacterial Resistance Ampicillin
Selectable Markers HIS3
Vector Type pRS423
Synonyms UBI3(1-76)
Antigen/Gene or Protein Targets Ubiquitin
Relevance Yeast episomal vector for expression of His-tagged ubiquitin; HIS3 marker. Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity).
Research Area Epigenetics & Nuclear Signalling, Metabolism, Neurobiology, Protein Degradation

References

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References: 1 entry

Detection of PCNA modifications in Saccharomyces cerevisiae. Davies AA, Ulrich HD Methods Mol Biol. 2012;920:543-67. doi: 10.1007/978-1-61779-998-3_36.


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References: 1 entry

Detection of PCNA modifications in Saccharomyces cerevisiae. Davies AA, Ulrich HD Methods Mol Biol. 2012;920:543-67. doi: 10.1007/978-1-61779-998-3_36.


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