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Anti-F-actin [NH3]

Invented by Dr Lynda Partridge at University Of Sheffield

Info

Catalogue Number 153598
Applications ELISA FACS
Antigen/Gene or Protein Targets Filamentous Actin
Synonyms Actin Antibody, Anti-Actin Antibody, F-Actin, f actin antibody, Filamentous actin antibody
Reactivity Chicken, Human, Mouse and Rabbit
Relevance Monoclonal antibody used to study the localisation and interactions of F-actin in all cell types.

Background and Research Application
Antibody NH3 has been shown to recognize actin. The epitope recognized is preserved across species (mouse, rabbit and human) and for different isoforms. NH3 recognises human filamentous actin (F-actin), but has been reported to recognise globular actin (G-actin).
NH3 therefore represents a useful probe for the localization of actin and the study of its interaction with other molecules. NH3 strongly stains the cytoplasm of macrophages and causes granular, localised nuclear staining of all cell types. NH3 also reacts with the cell surface of cell lines that originate from B-cells.
Host Mouse
Immunogen Human monocytes and the human monocyte-like cell line, U937.
Immunogen UniProt ID P60709
Subclass IgM kappa
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Studies suggest that the epitope may be located between residues 120 and 226 of actin, at the N-terminal region.

Concentration
1mg/ml as standard
Research Area Cell Type or Organelle Marker, Cell Structure and Motility, Developmental Biology

References

There are 1 reference entries for this reagent.

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References: 1 entry

Initial characterization of an anti-actin monoclonal antibody (NH3) DOI: 10.1042/bst0160163

ELISA FACS


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References: 1 entry

Initial characterization of an anti-actin monoclonal antibody (NH3) DOI: 10.1042/bst0160163

ELISA FACS


Add a reference