Anti-F-actin [NH3]
Invented by Dr Lynda Partridge from University Of Sheffield
Invented at University Of Sheffield
- Datasheet
- References (1)
- Inventor Info
Info
| Catalogue Number | 153598 |
| Applications | ELISA FACS |
| Antigen/Gene or Protein Targets | Filamentous Actin |
| Synonyms | Actin Antibody, Anti-Actin Antibody, F-Actin, f actin antibody, Filamentous actin antibody |
| Reactivity | Chicken, Human, Mouse and Rabbit |
| Relevance |
Monoclonal antibody used to study the localisation and interactions of F-actin in all cell types. Background and Research Application Antibody NH3 has been shown to recognize actin. The epitope recognized is preserved across species (mouse, rabbit and human) and for different isoforms. NH3 recognises human filamentous actin (F-actin), but has been reported to recognise globular actin (G-actin). NH3 therefore represents a useful probe for the localization of actin and the study of its interaction with other molecules. NH3 strongly stains the cytoplasm of macrophages and causes granular, localised nuclear staining of all cell types. NH3 also reacts with the cell surface of cell lines that originate from B-cells. |
| Host | Mouse |
| Immunogen | Human monocytes and the human monocyte-like cell line, U937. |
| Immunogen UniProt ID | P60709 |
| Subclass | IgM kappa |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Studies suggest that the epitope may be located between residues 120 and 226 of actin, at the N-terminal region. Concentration 1mg/ml as standard |
| Research Area | Cell Type or Organelle Marker, Cell Structure and Motility, Developmental Biology |
References: 1 entry
Initial characterization of an anti-actin monoclonal antibody (NH3) DOI: 10.1042/bst0160163
ELISA FACS
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References: 1 entry
Initial characterization of an anti-actin monoclonal antibody (NH3) DOI: 10.1042/bst0160163
ELISA FACS
Add a reference
