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- Inventor Info
|Antigen/Gene or Protein Targets||Filamentous Actin|
|Synonyms||Actin Antibody, Anti-Actin Antibody, F-Actin, f actin antibody, Filamentous actin antibody|
|Reactivity||Chicken, Human, Mouse and Rabbit|
Monoclonal antibody used to study the localisation and interactions of F-actin in all cell types.
Background and Research Application
Antibody NH3 has been shown to recognize actin. The epitope recognized is preserved across species (mouse, rabbit and human) and for different isoforms. NH3 recognises human filamentous actin (F-actin), but has been reported to recognise globular actin (G-actin).
NH3 therefore represents a useful probe for the localization of actin and the study of its interaction with other molecules. NH3 strongly stains the cytoplasm of macrophages and causes granular, localised nuclear staining of all cell types. NH3 also reacts with the cell surface of cell lines that originate from B-cells.
|Immunogen||Human monocytes and the human monocyte-like cell line, U937.|
|Immunogen UniProt ID||P60709|
Purified using multi-step affinity chromatography with protein A.
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.
Points of Interest
Studies suggest that the epitope may be located between residues 120 and 226 of actin, at the N-terminal region.
1mg/ml as standard
|Research Area||Cell Type or Organelle Marker, Cell Structure and Motility, Developmental Biology|
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