Floxed-Fbxw7 Mouse
Invented at University of Nottingham
Double Floxed Fbxw7/FoxM1
Invented at University of Nottingham
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 153594 |
| Antigen/Gene or Protein Targets | Fbxw7 and foxM1 |
| Disease Keywords | Colorectal cancer |
| Synonyms | F-box/WD repeat–containing protein 7, CDC4, Sel10, Ago, Fbw7, SCF, Skp1/Cullin/F-box protein, E3 ubiquitin ligase complex,FBW6, FBX30, FBXO30, FBXW6, SEL-10, hAgo, hCdc4, FOXM1, FKHL16, FOXM1B, HFH-11, HFH11, HNF-3, INS-1, MPHOSPH2, MPP-2, MPP2, PIG29, TGT3, TRIDENT, forkhead box M1 |
| Model | Conditional KO |
| Relevance |
FoxM1 is plays a key role in the cell cycle as endogenous expression peaks at the S and G2/M phases, crucial for mitotic division. Recent studies have shown that FOXM1 regulates expression of a large array of G2/M-specific genes which play a crucial role in maintenance of genomic stability and chromosomal segregation. There are 3 known isoforms of FoxM1, A, B & C and it is a known human proto-oncogene, involved in basal cell carcinoma. Fbxw7 is a member of the F-box protein family, characterised by a motif of approximately 40 amino acids called the F-box. These proteins constitute one of the four subunits of ubiquitin protein ligase complex called SKP1-cullin-F-box and function in phosphorylation-dependent ubiquitination. Fbxw7 specifically protein contains 7 tandem solenoid protein domains also know as WD40 repeats. Fbwx7 binds directly to cyclin E and probably targets it for ubiquitin-mediated degradation. |
| Production Details | This model was created by interbreeding the floxed-Fbxw7 mouse with a floxed-FoxM1 mouse (for floxed-FoxM1 mouse see references). |
| Conditional | Yes |
| Conditional Description | Requires expression of Cre recombinase to mediate excision of the lox P sites generating non-functional Fbxw7 and foxM1 proteins |
| Strain | C57BL/6 |
| Zygosity | Homozygous |
| Research Area | Cancer, Drug Discovery & Development, Metabolism |
References: 4 entries
Yoshida et al. 2007. Gastroenterology. 132(4):1420-31. PMID: 17408638.
The forkhead box M1 transcription factor contributes to the development and growth of mouse colorectal cancer.
Europe PMC ID: 17408638
Wang et al. 2002. Proc Natl Acad Sci U S A. 99(26):16881-6. PMID: 12482952.
The Forkhead Box m1b transcription factor is essential for hepatocyte DNA replication and mitosis during mouse liver regeneration.
Europe PMC ID: 12482952
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References: 4 entries
Yoshida et al. 2007. Gastroenterology. 132(4):1420-31. PMID: 17408638.
The forkhead box M1 transcription factor contributes to the development and growth of mouse colorectal cancer.
Wang et al. 2002. Proc Natl Acad Sci U S A. 99(26):16881-6. PMID: 12482952.
The Forkhead Box m1b transcription factor is essential for hepatocyte DNA replication and mitosis during mouse liver regeneration.
Add a reference
