Anti Human IgG [R10Z8E9]
Invented by Dr Margaret Goodall from University Of Birmingham
Invented at University Of Birmingham
- Datasheet
- References (19)
- Inventor Info
Info
| Catalogue Number | 153535 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Human immunoglobulin G - all subclasses |
| Synonyms | IgG, Immunoglobulin G, |
| Reactivity | N/A |
| Relevance |
Monoclonal antibody specific for human immunoglobulin G. Background and Research Application Antibodies make up a major component of humoral immunity, with Immunoglobulin G making up the main type found circulating in blood and extracellular fluid allowing permitting access to the body's tissues to control infection through binding pathogens like viruses, bacteria, and fungi. IgG is a type of antibody, has two antigen binding sites, and represents approximately 75% of serum antibodies in humans. IgG is the most common type of antibody found in the circulation and they are created and released by plasma B cells. This antibody is specific for human IgG. |
| Host | Mouse |
| Immunogen | Human IgM rheumatoid factor Waldenstrom’s macroglobulineamia |
| Immunogen UniProt ID | P01857 |
| Subclass | IgG1 |
| Myeloma Used | NS0 |
| Recommended Growing Conditions | RPMI 1640 supplemented with 10% Foetal bovine serum (bovine IgG depleted over SpA and PrG) and penicillin (100ug/ml). Cultured at 37oC in 5% CO2. |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Anti-IgG (R10Z8E9) has been demonstrated by ELISA not to recognise IgG from Non-Human Primate species (Baboon, Marmoset, Rhesus and Cynomologus macaques), nor does it recognise mouse or rat IgG. Concentration 1mg/ml as standard |
| Research Area | Cancer, Immunology |
References: 19 entries
Zhang et al. 2016. J Biol Chem. 291(16):8475-85. PMID: 26917724.
Laumet et al. 2015. Nat Neurosci. 18(12):1746-55. PMID: 26551542.
Zhang et al. 2015. J Biol Chem. 290(23):14647-55. PMID: 25925949.
Stubenrauch et al. 2010. J Pharm Biomed Anal. 52(2):249-54. PMID: 20083366.
Stubenrauch et al. 2010. Drug Metab Dispos. 38(1):84-91. PMID: 19850673.
Evaluation of a generic immunoassay with drug tolerance to detect immune complexes in serum samples from cynomolgus monkeys after administration of human antibodies.
Europe PMC ID: 20083366
Impact of molecular processing in the hinge region of therapeutic IgG4 antibodies on disposition profiles in cynomolgus monkeys.
Europe PMC ID: 19850673
Stubenrauch et al. 2009. J Pharm Biomed Anal. 49(4):1003-8. PMID: 19250787.
Evaluation of an immunoassay for human-specific quantitation of therapeutic antibodies in serum samples from non-human primates.
Europe PMC ID: 19250787
Yang et al. 2008. J Immunol Methods. 335(1-2):8-20. PMID: 18402977.
Quantitative determination of humanized monoclonal antibody rhuMAb2H7 in cynomolgus monkey serum using a Generic Immunoglobulin Pharmacokinetic (GRIP) assay.
Europe PMC ID: 18402977
Lund et al. 2000. Eur J Biochem. 267(24):7246-57. PMID: 11106438.
Expression and characterization of truncated forms of humanized L243 IgG1. Architectural features can influence synthesis of its oligosaccharide chains and affect superoxide production triggered through human Fcgamma receptor I.
Europe PMC ID: 11106438
Lund et al. 1996. J Immunol. 157(11):4963-9. PMID: 8943402.
Multiple interactions of IgG with its core oligosaccharide can modulate recognition by complement and human Fc gamma receptor I and influence the synthesis of its oligosaccharide chains.
Europe PMC ID: 8943402
Akinwolere et al. 1994. J Clin Pathol. 47(5):405-10. PMID: 8027392.
Two enzyme linked immunosorbent assays for detecting antibodies against meningococcal capsular polysaccharides A and C.
Europe PMC ID: 8027392
Hazlewood et al. 1993. Clin Exp Immunol. 93(2):157-64. PMID: 8348740.
The acquisition of anti-pneumococcal capsular polysaccharide Haemophilus influenzae type b and tetanus toxoid antibodies, with age, in the UK.
Europe PMC ID: 8348740
Add a reference
References: 19 entries
Zhang et al. 2016. J Biol Chem. 291(16):8475-85. PMID: 26917724.
Laumet et al. 2015. Nat Neurosci. 18(12):1746-55. PMID: 26551542.
Zhang et al. 2015. J Biol Chem. 290(23):14647-55. PMID: 25925949.
Stubenrauch et al. 2010. J Pharm Biomed Anal. 52(2):249-54. PMID: 20083366.
Stubenrauch et al. 2010. Drug Metab Dispos. 38(1):84-91. PMID: 19850673.
Evaluation of a generic immunoassay with drug tolerance to detect immune complexes in serum samples from cynomolgus monkeys after administration of human antibodies.
Impact of molecular processing in the hinge region of therapeutic IgG4 antibodies on disposition profiles in cynomolgus monkeys.
Stubenrauch et al. 2009. J Pharm Biomed Anal. 49(4):1003-8. PMID: 19250787.
Evaluation of an immunoassay for human-specific quantitation of therapeutic antibodies in serum samples from non-human primates.
Yang et al. 2008. J Immunol Methods. 335(1-2):8-20. PMID: 18402977.
Quantitative determination of humanized monoclonal antibody rhuMAb2H7 in cynomolgus monkey serum using a Generic Immunoglobulin Pharmacokinetic (GRIP) assay.
Lund et al. 2000. Eur J Biochem. 267(24):7246-57. PMID: 11106438.
Expression and characterization of truncated forms of humanized L243 IgG1. Architectural features can influence synthesis of its oligosaccharide chains and affect superoxide production triggered through human Fcgamma receptor I.
Lund et al. 1996. J Immunol. 157(11):4963-9. PMID: 8943402.
Multiple interactions of IgG with its core oligosaccharide can modulate recognition by complement and human Fc gamma receptor I and influence the synthesis of its oligosaccharide chains.
Akinwolere et al. 1994. J Clin Pathol. 47(5):405-10. PMID: 8027392.
Two enzyme linked immunosorbent assays for detecting antibodies against meningococcal capsular polysaccharides A and C.
Hazlewood et al. 1993. Clin Exp Immunol. 93(2):157-64. PMID: 8348740.
The acquisition of anti-pneumococcal capsular polysaccharide Haemophilus influenzae type b and tetanus toxoid antibodies, with age, in the UK.
Add a reference
