HCT 116 BRCA2 -/- [46] Cell Line
Invented at Cancer Research UK Cambridge Institute
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 153531 |
| Antigen/Gene or Protein Targets | BRCA2 |
| Parental Line | HCT 116 |
| Host | Human |
| Tissue | Colon |
| Disease Keywords | Colorectal carcinoma, cancer |
| Model | Knock-Out |
| Relevance |
Homozygous knockout cell line used to investigate the effects of BRCA2 mutations in a range of cancers. Background and Research Application BRCA2 -/- HCT116 human colorectal carcinoma cells were generated to study the role of BRCA2 in DNA repair and to identify additional functions of the BRCA2 gene. This includes the upregulation and effect of interferon-related genes (including APOBEC3F and APOBEC3G) when the BRAC2 gene is lost. This cell line shows phenotypes consistent with previous reports, including loss of Rad51 foci in the presence of double-strand breaks, chromosomal rearrangements and elevated sensitivity to the DNA-damaging agents phleomycin and Parp1 inhibitors. BRCA2 also has a role in transcriptional activation. BRAC2 inherited mutations, predispose carriers to various early onset cancers, including breast and ovarian. |
| Conditional | No |
| Research Area | Cancer, DNA Damage and Repair, Drug Discovery & Development |
| Notes |
Points of Interest Transcriptional activity of the IFN-stimulated response element (ISRE) was increased in BRCA2 knockout cells, and the expression of BRCA2 greatly decreased IFN-α stimulated ISRE reporter activity. This suggests that BRCA2 directly represses the expression of IFN-related genes through the ISRE. It has been found that there are two pathways regulating IFN-related genes in BRCA2−/− cells; one is the endogenous DNA damage in BRCA2−/− cells, the other is the direct transcriptional repression by BRCA2. The colony forming capacity of BRCA2 knockout cells was significantly reduced in the presence of either IFN-β or IFN-γ. This indicates a potential for IFN as therapeutic options in cases of BRCA2 mutations. This cell line has been used to show DNA damage occurs in BRCA2 knockout HCT116 cells even in the absence of exogenous genotoxic stress. HCT116 BRCA2-/- cells were created through targeted disruption of human BRCA2 by homologous recombination and deletion was confirmed by Southern Blot and Western Blot using antibodies against the C-terminus of the BRCA2 protein. Concentration Vial has between 1-5 million cells as standard, however this may vary. |
| Cellosaurus ID | CVCL_AS10 |
References: 4 entries
Xu et al. 2014. J Pathol. 234(3):386-97. PMID: 25043256.
Up-regulation of the interferon-related genes in BRCA2 knockout epithelial cells.
Europe PMC ID: 25043256
Issaeva et al. 2010. Cancer Res. 70(15):6268-76. PMID: 20631063.
Issaeva et al. 2010. Cancer Res. 70(15):6268-76. PMID: 20631063.
Add a reference
References: 4 entries
Xu et al. 2014. J Pathol. 234(3):386-97. PMID: 25043256.
Up-regulation of the interferon-related genes in BRCA2 knockout epithelial cells.
Issaeva et al. 2010. Cancer Res. 70(15):6268-76. PMID: 20631063.
Issaeva et al. 2010. Cancer Res. 70(15):6268-76. PMID: 20631063.
Add a reference
