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HCT 116 BRCA2 -/- [46] Cell Line

Info

Catalogue Number 153531
Antigen/Gene or Protein Targets BRCA2
Parental Line HCT 116
Host Human
Tissue Colon
Disease Keywords Colorectal carcinoma, cancer
Model Knock-Out
Relevance BRCA2-knockout HCT116 human colorectal carcinoma cells were generated by homologous recombination to study the role of BRCA2 in DNA repair and to identify additional functions of the BRCA2 gene. These cells show phenotypes consistent with previous reports, including loss of Rad51 foci in the presence of double-strand breaks, chromosomal rearrangements and elevated sensitivity to the DNA-damaging agents phleomycin and Parp1 inhibitors.
Conditional No
Research Area Cancer, DNA Damage and Repair, Drug Discovery & Development
Notes HCT116 BRCA2-/- cells were created through targeted disruption of human BRCA2 by homologous recombination and deletion was confirmed by Southern Blot and Western Blot using antibodies against the C-terminus of the BRCA2 protein.

This cell line was noted as clone 46 by the originating laboratory.
Cellosaurus ID CVCL_AS10

References

There are 2 reference entries for this reagent.

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References: 2 entries

Xu et al. 2014. J Pathol. 234(3):386-97. PMID: 25043256.

Up-regulation of the interferon-related genes in BRCA2 knockout epithelial cells.

Europe PMC ID: 25043256


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References: 2 entries

Xu et al. 2014. J Pathol. 234(3):386-97. PMID: 25043256.

Up-regulation of the interferon-related genes in BRCA2 knockout epithelial cells.


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