Anti-IgT [Z55F8*C3]
Invented at Vertebrate Antibodies Limited
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![Western blot showing detection of IgT in meagre (Argyrosomus regius) serum using Anti-IgT [Z55F8*C3]. Antibody was used neat (supernatant).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/66a4be2d-8a3b-41b2-83ae-45ed52ff1b2e.png)
![Western blot showing detection of IgT in sera of gilt-head (sea) bream (Sparus aurata) Lumpsucker (Cyclopteridae) and Large yellow croaker (Larimichthys crocea) using Anti-IgT [Z55F8*C3].](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/81d8d9b0-8748-4729-b828-50dcc96f9c6f.png)
![Immunohistochemistry was performed on formalin-fixed paraffin-embedded tissues. Positive membranous immunostaining is observed in Meagre gill tissue using Anti-IgT [Z55F8*C3]. Antigen retrieval step is required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/2e158121-ac82-453f-bd24-ad9186291280.png)
![Western blot showing detection of IgT in meagre (Argyrosomus regius) serum using Anti-IgT [Z55F8*C3]. Antibody was used neat (supernatant).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/66a4be2d-8a3b-41b2-83ae-45ed52ff1b2e.png)
![Western blot showing detection of IgT in sera of gilt-head (sea) bream (Sparus aurata) Lumpsucker (Cyclopteridae) and Large yellow croaker (Larimichthys crocea) using Anti-IgT [Z55F8*C3].](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/81d8d9b0-8748-4729-b828-50dcc96f9c6f.png)
![Immunohistochemistry was performed on formalin-fixed paraffin-embedded tissues. Positive membranous immunostaining is observed in Meagre gill tissue using Anti-IgT [Z55F8*C3]. Antigen retrieval step is required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/2e158121-ac82-453f-bd24-ad9186291280.png)
![Image thumbnail for Anti-IgT [Z55F8*C3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/66a4be2d-8a3b-41b2-83ae-45ed52ff1b2e.png)
![Image thumbnail for Anti-IgT [Z55F8*C3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/81d8d9b0-8748-4729-b828-50dcc96f9c6f.png)
![Image thumbnail for Anti-IgT [Z55F8*C3]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/2e158121-ac82-453f-bd24-ad9186291280.png)
| Catalogue Number | 153529 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | IgT, Immunoglobulin Heavy Constant Tau |
| Synonyms | IgT |
| Reactivity | Gilthead seabream, Meagre and Lumpsucker |
| Relevance |
Anti-IgT antibody with use to develop vaccines, in fish. Background and Research Application Immunoglobulin T (IgT) is one of the key effector molecules of jawed vertebrate's adaptive immune system, and acts like a mucosal antibody. The target species of this antibody include: Meagre (Argyrosomus regius), gilt-head (sea) bream (Sparus aurata), Lumpsucker (Cyclopteridae), Large yellow croaker (Larimichthys crocea). This anti-IgT antibody provides a useful tool to monitor vaccine performance in fish and will assist in the development of future vaccines, especially mucosal vaccines. |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptide |
| Positive Control | ELISA - fish sera, peptide immunogen; Western Blot, Sera; IHC, Formalin-fixed, paraffin-embedded head kidney, gill and spleen tissues |
| Subclass | IgG |
| Myeloma Used | P3X63Ag8.653 |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Detection of specific antibodies in the serum of animals is an indicator of previous exposure to pathogens which is very valuable for brood stock health testing. This antibody has been used to investigate quantitative distribution of IgT-expressing and IgT-producing cells in tissues of the European seabass Dicentrarchus labrax. Recommended Usage Conditions: ELISA- neat; Western blot- neat; IHC- 1/5 dilution, antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0 Concentration 1mg/ml as standard |
| Research Area | Aquaculture |
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