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Anti-HuD/HuC/HuB [16A11]

Info

Catalogue Number 153503
Applications ELISA IHC IF WB
Antigen/Gene or Protein Targets HuD
Synonyms ELAV-like protein 4
Reactivity Human
Relevance Monoclonal antibody which recognises HuD, HuC and HuB neuronal proteins.

Background and Research Application
HuD otherwise known as ELAV-like protein 4 is a protein that in humans is encoded by the ELAVL4 gene. The HuD/ELAVL4 protein is an RNA-binding protein. Diseases associated with ELAVL4 include Lambert-Eaton Myasthenic Syndrome and Sensory Peripheral Neuropathy. HuD is expressed only in neurons and it binds to AU-rich element-containing mRNAs. As a result of this interaction the half-life of the transcript is increased. HuD is important in neurons during brain development and plasticity.

The 16A11 anti-HuD monoclonal is now a classic reagent for the identification of neurons. Antibody 16A11 recognizes the ELAV family members HuC, HuD and Hel-N1 neuronal proteins. It does not recognize HuR, another ELAV family member that is present in all proliferating cells. The antibody has been shown to specifically label neuronal cells in zebrafish, chick, canaries, and humans, and is likely to label neuronal cells in most vertebrate species.
Host Mouse
Immunogen Peptide
Immunogen UniProt ID P26378
Subclass IgG2b
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Labelling is visible early in development, at about the time that the neurons leave the mitotic cycle.

Concentration
1mg/ml as standard
Research Area Developmental Biology, Gene Expression, Neurobiology

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