Cancer Research Technology
Log in Register
Menu
Search
Monoclonal Antibodies

Anti-Lamin B receptor [BB2SS3F3]

Invented at A*STAR Accelerate Technologies Pte Ltd
  • Datasheet
  • Inventor Info
Print

Info

Image thumbnail for Anti-Lamin B receptor [BB2SS3F3]
Image thumbnail for Anti-Lamin B receptor [BB2SS3F3]
Image thumbnail for Anti-Lamin B receptor [BB2SS3F3]
Image thumbnail for Anti-Lamin B receptor [BB2SS3F3]
Add an image
Western blot analysis of BB2SS3F3 antibody. Whole cell lysate from human (Hela) and mouse (C2C12, immortalized mouse adult fibroblasts (MAFs), NIE-115) cell lines were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and blotted with BB2SS3F3 antibody. Both primary and secondary antibodies were diluted in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). The predicted molecular weight is 70.7kDA and 71.4kDa for human and mouse LBR respectively. The absence of a band in LBR null MAFs indicated that the antibody specifically recognizes LBR.
Immunofluorescence staining of mouse small intestine. Small intestine from adult C57BL/6 mice was transferred to OCT compound and frozen in liquid nitrogen. Tissue cryosections were fixed in 10% neutral buffered formalin, permeabilized and stained with anti-LBR BB2SS3F3 antibody.
Confocal images of human (Hela) and mouse (NIE-115) cell lines immunofluorescently stained with BB2SS3F3 antibody. The BB2SS3F3 antibody works on cells fixed with 4% PFA and cold methanol with methanol fixation resulting in brighter signal of the antibody.
The antibody specifically detects LBR. Wild-type immortalized mouse adult fibroblast (MAFs LBR+/+) (upper panel) and LBR-null MEFs (MAFs LBR-/-) (lower panel) were methanol fixed and immunofluorescently stained with BB2SS3F3 mouse monoclonal antibody. In LBR-null MEFs there is a loss of BB2SS3F3 antibody staining signal indicating the LBR specific signal of the BB2SS3F3 antibody.
Add an image
Image thumbnail for Anti-Lamin B receptor [BB2SS3F3] Image thumbnail for Anti-Lamin B receptor [BB2SS3F3] Image thumbnail for Anti-Lamin B receptor [BB2SS3F3] Image thumbnail for Anti-Lamin B receptor [BB2SS3F3]
Catalogue Number 153492
Applications IHC IF WB
Antigen/Gene or Protein Targets Lamin B receptor
Synonyms DHCR 14B antibody, DHCR14B antibody, Integral nuclear envelope inner membrane protein antibody, Lamin-B receptor antibody, LBR antibody, LBR_HUMAN antibody, LMN 2R antibody, LMN2R antibody, MGC9041 antibody, PHA antibody, PRO0650 antibody
Reactivity Human and Mouse
Relevance Lamin B Receptor (LBR) is a polytopic inner nuclear membrane protein. Its nucleoplasmic domain interacts with components of the nuclear lamina as well as with chromatin, via HP1. LBR also exhibits C14 sterol reductase activity. Deficiency of LBR in humans results in Pelget-Huët anomaly featuring hypolobulated granulocyte nuclei. Homozygous loss of LBR causes Greenberg skeletal dysplasia a fetal lethal disorder associated with defective cholesterol metabolism.
Host Mouse
Immunogen GST fused to N-terminal fragment of human LBR (amino acids residues 1-211)
Positive Control Hela, C2C12, NIE-116
Subclass IgG1 kappa
Myeloma Used Sp2/0-Ag14
Strain C57BL/6
Notes Hybridoma cell line monoclonality was achieved by single cell sorting using flow cytometry. Human LBR is 70,7kDa and mouse is 71.4kDa. Entrez Gene Number is 3930. Gene symbol is LBR. UniProt Number is Q14739. Immunohistochemistry: paraffin-embedded section, cryosections. Immunocytochemistry/Immunofluorescence: formaldehyde, methanol fixation with methanol fixation resulting in a brighter signal.
Research Area Cell Signaling & Signal Transduction

References

There are 0 reference entries for this reagent.

References: 0 entry

There is no reference for this reagent yet, feel free to use the button below to suggest one.

Add a reference

References: 0 entry

There is no reference for this reagent yet, feel free to use the button below to suggest one.

Add a reference

Inventor Information

No inventors are currently linked to this reagent.

Add an inventor

Invented at

A*STAR Accelerate Technologies Pte Ltd
A*STAR Accelerate Technologies Pte Ltd

73 reagents listed on Ximbio