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Monoclonal Antibodies

Anti-Lamin B receptor [BB3SS4B5]

Invented at A*STAR Accelerate Technologies Pte Ltd
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Image thumbnail for Anti-Lamin B receptor [BB3SS4B5]
Image thumbnail for Anti-Lamin B receptor [BB3SS4B5]
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Western blot analysis of BB3SS4B5 antibody. Whole cell lysate from human (Hela) and mouse (C2C12, mouse adult fibroblasts (MAFs)) cell lines cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and blotted with BB3SS4B5 antibody. Both primary and secondary antibodies were diluted in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). The predicted molecular weight is 70.7kDA and 71.4kDa for human and mouse LBR respectively. The absence of a band in LBR null MAFs indicated that the antibody specifically recognizes LBR.
Immunofluorescence staining of mouse small intestine. Small intestine from adult C57BL/6 mice was transferred to OCT compound and frozen in liquid nitrogen. Tissue cryosections were fixed in 10% neutral buffered formalin, permeabilized and stained with anti-LBR BB3SS4B5 antibody. The antibody also works on paraffin-embedded section.
Immunofluorescence staining of human (Hela) and mouse (C2C12) cell lines with BB3SS4B5 antibody. Confocal images of Hela and C2C12 cell fixed and stained with mouse monoclonal BB3SS4B5 antibody. The BB3SS4B5 antibody works on cells fixed with 4% PFA and cold methanol with methanol fixation resulting in a brighter signal of the antibody.
The antibody specifically detects LBR. (A) 3T3 cells stably expressing mCherry-tagged mouse LBR were fixed in methanol and stained with BB3SS4B5 antibody. The expression levels of mCherry-LBR correspond to intensity of BB3SS4B5 antibody staining. (B) Wild-type mouse adult fibroblast (MAFs LBR+/+) (upper panel) and LBR-null MEFs (MAFs LBR-/-) (lower panel) were methanol fixed and immunofluorescently stained with BB3SS4B5 mouse monoclonal antibody. In LBR-null MEFs there is a loss of BB3SS4B5 antibody staining signal.
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Catalogue Number 153491
Applications IHC IF WB
Antigen/Gene or Protein Targets Lamin B receptor
Synonyms DHCR 14B antibody, DHCR14B antibody, Integral nuclear envelope inner membrane protein antibody, Lamin-B receptor antibody, LBR antibody, LBR_HUMAN antibody, LMN 2R antibody, LMN2R antibody, MGC9041 antibody, PHA antibody, PRO0650 antibody
Reactivity Human and Mouse
Relevance Lamin B Receptor (LBR) is a polytopic inner nuclear membrane protein. Its nucleoplasmic domain interacts with components of the nuclear lamina as well as with chromatin, via HP1. LBR also exhibits C14 sterol reductase activity. Deficiency of LBR in humans results in Pelget-Huët anomaly featuring hypolobulated granulocyte nuclei. Homozygous loss of LBR causes Greenberg skeletal dysplasia a fetal lethal disorder associated with defective cholesterol metabolism.
Host Mouse
Immunogen GST fused to N-terminal fragment of human LBR (amino acids residues 1-211)
Positive Control Hela, C2C12, NIE-115
Subclass IgG1 kappa
Myeloma Used Sp2/0-Ag14
Strain C57BL/6
Notes Hybridoma cell line monoclonality was achieved by single cell sorting using flow cytometry. Human LBR is 70,7kDa and mouse is 71.4kDa. Entrez Gene Number is 3930. Gene symbol is LBR. UniProt Number is Q14739. Immunohistochemistry: paraffin-embedded section, cryosections. Immunocytochemistry/Immunofluorescence: formaldehyde, methanol fixation with methanol fixation resulting in a brighter signal.
Research Area Cell Signaling & Signal Transduction

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Invented at

A*STAR Accelerate Technologies Pte Ltd
A*STAR Accelerate Technologies Pte Ltd

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