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Monoclonal Antibodies

Anti-Lamin A+C [WL4G10]

Invented at A*STAR Accelerate Technologies Pte Ltd
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Image thumbnail for Anti-Lamin A+C [WL4G10]
Image thumbnail for Anti-Lamin A+C [WL4G10]
Image thumbnail for Anti-Lamin A+C [WL4G10]
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Western blot analysis of WL4G10 antibody. Whole cell lysate from Hela cells and 293T cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and stained with WL4G10 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. As expected 293T cells express less of the lamin A isoform.
Confocal images of methanol fixed Hela cells that were immunofluorescently stained with mouse monoclonal WL4G10 antibody. The antibody works as well on cells fixed with 4% PFA.
The mouse monoclonal WL4G10 antibody recognizes human lamins A and C. Immortalized mouse adult LMNA null fibroblast stably expressing: (A) mEos2-lamin A or (B) mEos2- lamin C fused were fixed in 4% PFA and stained with WL4G10 antibody (magenta). Expression of the respective LMNA isoform is shown in green. The nuclei were counter-stained with Hoechst. Of note the WL4G10 antibody does not recognize mouse lamins A and C.
Immunofluorescence analysis of formalin-fixed paraffin-embedded human gut. Following heat induced antigen retrieval (Dako antigen retrieval solution pH6 or pH9) the sections were stained with mouse monoclonal anti-lamin A+C clone WL4G10 antibody (green). Nuclei were visualized with DAPI.
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Image thumbnail for Anti-Lamin A+C [WL4G10] Image thumbnail for Anti-Lamin A+C [WL4G10] Image thumbnail for Anti-Lamin A+C [WL4G10] Image thumbnail for Anti-Lamin A+C [WL4G10]
Catalogue Number 153490
Applications IHC IF WB
Antigen/Gene or Protein Targets Lamin A and Lamin C
Synonyms 71 kDa lamin antibody, Cardiomyopathy dilated 1A (autosomal dominant) antibody, CDCD1 antibody, CDDC antibody, CMD1A antibody, CMT2B1 antibody, EMD2 antibody, FPL antibody, FPLD antibody, FPLD2 antibody, HGPS antibody, IDC antibody, Lamin A antibody, Lamin A/C antibody, Lamin A/C like 1 antibody, Lamin antibody, Lamin C antibody, Lamin-A/C antibody, LDP1 antibody
Reactivity Human
Relevance Lamins A and C are major components of the nuclear lamina, a thin protein meshwork located at the nuclear face of the nuclear envelope (NE). The lamina provides structural integrity to the NE and is involved in many other aspects of nuclear biology including transcription and chromatin organization. Lamins A and C arise from the LMNA gene by alternative splicing and majority of adult tissues express at least one of the isoform. The LMNA gene has been extensively studies due to its association with variety of human diseases. Mutations in LMNA have been linked 12 distinct disorders, including Emery-Dreifus muscular dystrophy, dilated cardiomyopathy, Dunnigan-type partial lipodystrophy and Hutchinson-Gilford progeria syndrome.
Host Mouse
Immunogen GST fused to C-terminal fragment of human lamin A (amino acids residues 422-664)
Positive Control Hela, 293T
Subclass IgG1 kappa
Myeloma Used Sp2/0-Ag14
Strain C57BL/6
Notes Lamin A is 74kDa and Lamin C is 65 kDa. Entrez Gene Number is 4000. Gene symbol is LMNA. UniProt Number is P02545. Immunohistochemistry: paraffin-embedded section, cryosections. Immunocytochemistry/Immunofluorescence: formaldehyde, methanol fixation.
Research Area Cell Signaling & Signal Transduction

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Invented at

A*STAR Accelerate Technologies Pte Ltd
A*STAR Accelerate Technologies Pte Ltd

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