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Monoclonal Antibodies

Anti-Lamin A+C [LASS2D9]

Invented at A*STAR Accelerate Technologies Pte Ltd
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Image thumbnail for Anti-Lamin A+C [LASS2D9]
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Western blot analysis of LASS2D9 antibody. Whole cell lysate from human (Hela) and mouse (C2C12, mouse adult fibroblasts (MAFs)) cell lines cells were separated on SDS-PAGE gel, transferred to nitrocellulose membrane and blotted with LASS2D9 antibody. The membrane was blocked overnight in blocking buffer (5% nonfat dry milk resuspended in 0.1% Tween and 1X PBS). Both primary and secondary antibodies were diluted in blocking buffer. The predicted molecular weight for lamin A is 74 kDA and for lamin C 65 kDa. The absence of a band in LMNA null MAFs indicated that the antibody specifically recognizes products of LMNA gene.
Immunofluorescence staining of mouse small intestine. Small intestine from adult C57BL/6 mice was transferred to OCT compound and frozen in liquid nitrogen. Tissue cryosections were fixed in 10% neutral buffered formalin, permeabilized and stained with anti-lamin A+C LASS2D9 antibody. The antibody also works on paraffin-embedded section.
Immunofluorescence staining of human (Hela) and mouse (C2C12) cell lines with LASS2D9 antibody. Confocal images of Hela and C2C12 cell fixed and stained with mouse monoclonal LASS2D9 antibody. The LASS2D9 antibody works on cells fixed with 4% PFA and cold methanol.
The mouse monoclonal LASS2D9 antibody specifically detects lamin A and lamin C. (A) Wild-type mouse adult fibroblast (MAFs LMNA+/+) (upper panel) and LMNA-null MEFs (MAFs LMNA-/-) (lower panel) were methanol fixed and immunofluorescently stained with LASS2D9 mouse monoclonal antibody. (B) LMNA-null mouse adult fibroblast stably expressing mEos2-lamin A (upper panel) or mEos2-lamin C (lower panel) were fixed in cold methanol and stained with LASS2D9 antibody (magenta). Expression of the respective LMNA isoform is shown in green. The nuclei were counterstained with Hoechst. In LMNA-null MEFs there is a loss of LASS2D9 antibody staining signal. Rescue of LMNA-null cells with lamin A or lamin C expressing constructs restores the LASS2D9 antibody signal strongly suggesting that LASS2D9 specifically detects the products of LMNA gene.
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Catalogue Number 153489
Applications IHC IF WB
Antigen/Gene or Protein Targets Lamin A and Lamin C
Synonyms 70 kDa lamin antibody, Cardiomyopathy dilated 1A (autosomal dominant) antibody, CDCD1 antibody, CDDC antibody, CMD1A antibody, CMT2B1 antibody, EMD2 antibody, FPL antibody, FPLD antibody, FPLD2 antibody, HGPS antibody, IDC antibody, Lamin A antibody, Lamin A/C antibody, Lamin A/C like 1 antibody, Lamin antibody, Lamin C antibody, Lamin-A/C antibody, LDP1 antibody,
Reactivity Human and Mouse
Relevance Lamins A and C are major components of the nuclear lamina, a thin protein meshwork located at the nuclear face of the nuclear envelope (NE). The lamina provides structural integrity to the NE and is involved in many other aspects of nuclear biology including transcription and chromatin organization. Lamins A and C arise from the LMNA gene by alternative splicing and majority of adult tissues express at least one of the isoform. The LMNA gene has been extensively studies due to its association with variety of human diseases. Mutations in LMNA have been linked 12 distinct disorders, including Emery-Dreifus muscular dystrophy, dilated cardiomyopathy, Dunnigan-type partial lipodystrophy and Hutchinson-Gilford progeria syndrome.
Host Mouse
Positive Control Hela, 293T, C2C12, fibroblasts
Subclass IgG3 kappa
Myeloma Used Sp2/0-Ag14
Strain C57BL/6
Notes Lamin A is 74kDa and Lamin C is 65 kDa. Entrez Gene Number is 4000. Gene symbol is LMNA. UniProt Number is P02545. Immunohistochemistry: paraffin-embedded section, cryosections. Immunocytochemistry/Immunofluorescence: formaldehyde, methanol fixation.
Research Area Cell Signaling & Signal Transduction

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Invented at

A*STAR Accelerate Technologies Pte Ltd
A*STAR Accelerate Technologies Pte Ltd

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