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Monoclonal Antibodies

Anti-SUN1 [12.10F]

Invented at A*STAR Accelerate Technologies Pte Ltd
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Confocal immunofluorescence images of mouse C2C12 cell line fixed with either cold methanol (MeOH) (upper panel) or 4% paraformaldehyde (4% PFA) (lower panel) and stained with 12.10F antibody. Nuclei were visualised with Hoechst.
The antibody specifically detects Sun1. Immortalized wild-type mouse adult fibroblast (MAFs Sun1+/+) (upper panel) and Sun1-null MAFs (Sun1-/-) (lower panel) were fixed in cold MeOH and immunofluorescently stained for Sun1 with 12.10F mouse monoclonal antibody.
Immunohistochemical staining of mouse tissues with anti-Sun1 12.10F antibody. (A) Testis was embedded in OCT compound and frozen in liquid nitrogen. Tissue cryosections were fixed in 10% neutral buffered formalin, incubated in 1% Triton X-100 (in PBS) and stained with anti-Sun1 12.10F antibody. (B) Skeletal muscle was embedded in paraffin and sectioned. Following heat-induced antigen retrieval (sodium citrate buffer pH 6) the paraffin sections were incubated with 1% Triton X-100 (in PBS) and subsequently stained with mouse monoclonal 12.10F antibody (lower panel). In both cases nuclei were visualised with Hoechst.
Western blot analysis of anti-Sun1 clone 12.10F monoclonal antibody. Nuclear extract from C2C12 at different stages of differentiation were separated on 8% SDS-PAGE gel, transferred to a PDVF membrane and blotted with anti-Sun1 of 12.10F antibody. Primary and secondary antibodies were diluted in washing buffer (1X TBS with 0.1% Tween). The predicted molecular weight of the longest mouse Sun1 isoform is 102 kDa. Note that several Sun1 isoforms exists.
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Catalogue Number 153483
Applications IF WB
Antigen/Gene or Protein Targets SUN-1
Synonyms Protein unc-84 homolog A antibody, Sad1 and UNC84 domain containing 1 antibody, Sad1/unc 84 protein like 1 antibody, Sad1/unc-84 protein-like 1 antibody, Sun domain-containing protein 1 antibody, Sun1 antibody, SUN1_HUMAN antibody
Reactivity Mouse and Rat
Relevance A notable feature of metazoan cells is their morphological plasticity associated with acquisition of a range of complex activities. Implementation of numerous specialized functions during differentiation is dependent upon the rearrangement and transformation of organelles, including the cell nucleus. Crucial to many cell specific changes in nuclear localization and organization is establishment of connections between the nucleus and cytoskeleton. Such connections are formed in part by KASH-domain proteins of the outer nuclear membrane (ONM). In mammals six KASH proteins are known (Nesprins 1, 2, 3, 4, LRMP and KASH5). Appropriate localization of KASH proteins is dependent upon tethering in the ONM by SUN-domain proteins of the inner nuclear membrane. Together, SUN-KASH pairs form LINC complexes (LInker of the Nucleoskeleton and Cytoskeleton) that span both nuclear membranes and which couple nuclear structures to the cytoskeleton. In recent years it has become apparent that LINC complex defects may contribute to a number of human genetic diseases,
including muscular dystrophy, cerebellar ataxia, hearing loss and infertility.

Sun1 is one of at least five mammalian SUN protein family members and is ubiquitously expressed. Of note, Sun1 undergoes extensive alternative splicing. Human and mouse tissues express simultaneously several isoforms with the presence and prevalence of specific isoforms being tissue dependent.
Host Mouse
Immunogen GST fused to residues encoded by exons 6, 8, 10
Immunogen UniProt ID Q9D666
Positive Control C2C12, mouse fibroblast
Subclass IgG2a kappa
Molecular Weight (kDa) 102
Myeloma Used Sp2/0-Ag14
Strain C57BL/6
Notes Species Reactivity: Mouse, does not react with human; not tested with other species
Epitope: Not known
Knock Out Validated

Entrez Gene Number: 77053
Gene Symbol: KIAA0810, UNC84A
UniProt Number: Q9D666
Molecular Weight: Mouse 102 kDa; existence of multiple shorter isoforms
Research Area Cell Signaling & Signal Transduction, Developmental Biology

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Invented at

A*STAR Accelerate Technologies Pte Ltd
A*STAR Accelerate Technologies Pte Ltd

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