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Monoclonal Antibodies

Anti-Nesprin-2 [10H8]

Invented at A*STAR Accelerate Technologies Pte Ltd
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HEK293 cells transiently expressing either GFP alone or GFP fused a small Nesp2 isoform (Nesp2a2DKASH) lacking the C-terminal KASH domain. This small isoform contains the ~180 residue Nesp2 polypeptide that was employed in the immunogen. Total cell lysates were analyzed by western blot employing either a rabbit anti-GFP or culture supernatant from three independent anti-Nesp2 hybridoma clones (6C11, 10H8 and 5C3)
Immunofluorescence microscopy of HeLa cells labeled with either 6C11 or 10H8 monoclonal antibodies raised against human Nesp2. The antibodies can be used on either formaldehyde- or methanol-fixed cells.
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Catalogue Number 153481
Applications IF WB
Antigen/Gene or Protein Targets Nesprin-2
Synonyms Nesp2 antibody, Nesprin-2 antibody, Nuclear envelope spectrin repeat protein 2 antibody
Reactivity Human
Relevance A notable feature of metazoan cells is their morphological plasticity associated with acquisition of a range of complex activities. Implementation of numerous specialized functions during differentiation is dependent upon the rearrangement and transformation of organelles, including the cell nucleus. Crucial to many cell specific changes in nuclear localization and organization is establishment of connections between the nucleus and cytoskeleton. Such connections are formed in part by KASH-domain proteins of the outer nuclear membrane (ONM). In mammals six KASH proteins are known (Nesprins 1, 2, 3, 4, LRMP and KASH5). Appropriate localization of KASH proteins is dependent upon tethering in the ONM by SUN-domain proteins of the inner nuclear membrane. Together, SUN-KASH pairs form LINC complexes (LInker of the Nucleoskeleton and Cytoskeleton) that span both nuclear membranes and which couple nuclear structures to the cytoskeleton. In recent years it has become apparent that LINC complex defects may contribute to a number of human genetic diseases,
including muscular dystrophy, cerebellar ataxia, hearing loss and infertility.
Host Mouse
Immunogen GST fused to residues 6,653-6,835 of human Nesp2
Subclass IgG2b kappa
Myeloma Used Sp2/0-Ag14
Strain C57BL/6
Research Area Developmental Biology, Gene Expression

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Invented at

A*STAR Accelerate Technologies Pte Ltd
A*STAR Accelerate Technologies Pte Ltd

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