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LAIR-1 KO Mouse

Info

Catalogue Number 153476
Antigen/Gene or Protein Targets Leukocyte-associated immunoglobulin-like receptor-1
Disease Keywords Thrombocytosis
Synonyms Leukocyte-associated immunoglobulin-like receptor-1, LAIR-1, LAIR1, CD305, cluster of differentiation 305,
Model Knock-Out
Relevance Leukocyte-associated immunoglobulin-like receptor-1, or LAIR-1, is a collagen receptor expressed on hematopoietic cells which can differentiate to peripheral mononuclear cells like NK, T and B cells. LAIR-1 is also expressed on immature megakaryocytes but undetectable on platelets, the physiological role that LAIR-1 plays on megakaryocytes and platelet formation is currently poorly understood.

LAIR-1 belongs to the inhibitory immunoreceptor tyrosine-based inhibition motif–containing receptor family. The inhibitory receptor regulates the immune response preventing lysis of cells recognized as self. LAIR-1 inhibits signalling via the immunoreceptor tyrosine-based activation motif-containing receptor complex, glycoprotein VI-FcRγ-chain.

The gene which encodes LAIR-1 is encoded in the leukocyte receptor cluster which also encodes at least 29 other genes encoding leukocyte-expressed receptors from the immunoglobulin superfamily.

LAIR-1 is known to interact with PTPN11 and PTPN6.
Breeding Information The mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
Conditional Description The proximal promoter and exons 1 and 2 of LAIR-1 were replaced with a flanked positive puromycin selection cassette expressed under the control of a eukaryotic promoter and includes a polyadenylation signal. Homologous recombinant clones were isolated using Puromycin resistance. The BAC targeting vector used was from the C57BL/6J RPCIB-731 BAC library and was transfected into the TaconicArtemis C57BL/6N Tac ES cell line. Removal of the promoter and exons 1 and 2 deletes the translation initiation codon and the sequence encoding the signal peptide. In addition the absence of the 5’UTR should also prevent transcription of the messenger RNA. Flp-mediated removal of the positive selection cassette also results in a constitutive KO allele.
Zygosity Homozygous
Research Area Cell Signaling & Signal Transduction, Immunology

References

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References: 2 entries

Smith et al. 2017. Arterioscler Thromb Vasc Biol. 37(5):823-835. PMID: 28336561.

Mice Lacking the Inhibitory Collagen Receptor LAIR-1 Exhibit a Mild Thrombocytosis and Hyperactive Platelets.

Europe PMC ID: 28336561


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References: 2 entries

Smith et al. 2017. Arterioscler Thromb Vasc Biol. 37(5):823-835. PMID: 28336561.

Mice Lacking the Inhibitory Collagen Receptor LAIR-1 Exhibit a Mild Thrombocytosis and Hyperactive Platelets.


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