Anti-PIWIL3 [Z83]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
- Datasheet
- References (0)
- Inventor Info
Info
![Western blot showing detection of PIWIL3 in (A) Jurkat and (B) Hela cell lysates using Anti-PIWIL3 [Z83]. Antibody was used at 1 in 2 dilution.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/ee1544ff-5dc5-426a-9320-fd51d6094a8b.png)
![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections. Positive cytoplasmic staining was observed in prostate cancer sections using Anti-PIWIL3 [Z83]. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/f8f8add8-f274-4de8-b75a-eff45b2aa656.jpg)
![Western blot showing detection of PIWIL3 in (A) Jurkat and (B) Hela cell lysates using Anti-PIWIL3 [Z83]. Antibody was used at 1 in 2 dilution.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/ee1544ff-5dc5-426a-9320-fd51d6094a8b.png)
![Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections. Positive cytoplasmic staining was observed in prostate cancer sections using Anti-PIWIL3 [Z83]. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/f8f8add8-f274-4de8-b75a-eff45b2aa656.jpg)
![Image thumbnail for Anti-PIWIL3 [Z83]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/ee1544ff-5dc5-426a-9320-fd51d6094a8b.png)
![Image thumbnail for Anti-PIWIL3 [Z83]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/f8f8add8-f274-4de8-b75a-eff45b2aa656.jpg)
| Catalogue Number | 153471 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Piwi Like RNA-Mediated Gene Silencing 3 |
| Synonyms | Anti-PIWIL3 antibody, HIWI3 antibody, Piwi like 3 antibody, PIWL3_HUMAN antibody |
| Reactivity | Human |
| Relevance | PIWIL3 may play a role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. PIWIL3 acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. PIWIL3 directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs may be involved in other processes during meiosis such as translation regulation. |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptide PNRSLSTRLFYL- peptide immunogen is identical in primates |
| Positive Control | IHC- formalin-fixed, paraffin-embedded multi tumour tissue microarray; Western Blot - Jurkat and Hela cell lysates |
| Subclass | IgG |
| Myeloma Used | P3X63Ag8.653 |
| Strain | Balb/c |
| Notes | Recommended Usage Conditions: ELISA - neat, IHC- antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0; Western blot - 1 in 2 dilution |
| Research Area | Developmental Biology, Epigenetics & Nuclear Signalling, Stem Cell Biology |
References
There are 0 reference entries for this reagent.
References: 0 entry
There is no reference for this reagent yet, feel free to use the button below to suggest one.
Add a reference
References: 0 entry
There is no reference for this reagent yet, feel free to use the button below to suggest one.
Add a reference
