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Venus-Mad2 Reporter Cell Line [RPE1 Venus-Mad2/+ KI clone #1]

Invented by Dr Jonathon Pines at Gurdon Institute

Info

Catalogue Number 153465
Antigen/Gene or Protein Targets Mad2
Parental Line RPE1
Synonyms MAD2L1
Host Human
Tissue Eye
Model Reporter
Relevance Somatic knock-in reporter cell line with endogenous expression of Mad2 fused to the yellow fluorescent protein Venus (Venus-Mad2 fusion protein). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cell line functions as a cell cycle reporter across the metaphase-to-anaphase transition of the cell cycle. See also the Cyclin A2 and Cyclin B1 versions of these cell lines for a broader analysis of the different phases of the cell cycle. This cell line is an endogenous reporter for Mad2 levels and activity.
Production Details To generate the cell line recombinant adenovirus-associated virus (rAVV)-mediated gene targeting was used to fuse the yellow fluorescent protein (Venus) ORF to the 5' of exon 1 of one allele of the MAD2L1 gene. The Mad2 was tagged at its N-terminus because a GFP-Mad2 fusion protein had previously been demonstrated to be functional in Drosophila.
Conditional No
Research Area Cell Cycle
Recommended Growing Conditions F12/DMEM (Sigma: D6241) + 2mM GlutaMAX(Invitrogen), 10% Foetal Bovine Serum (FBS) + 0.348% sodium bicarbonate; 37oC; 5% CO2.

References

There are 2 reference entries for this reagent.

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References: 2 entries

Collin et al. 2013. Nat Cell Biol. 15(11):1378-85. PMID: 24096242.

The spindle assembly checkpoint works like a rheostat rather than a toggle switch.

Europe PMC ID: 24096242


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References: 2 entries

Collin et al. 2013. Nat Cell Biol. 15(11):1378-85. PMID: 24096242.

The spindle assembly checkpoint works like a rheostat rather than a toggle switch.


Add a reference