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Catalogue Number 153460
Parental Line Subglottic brushing specimen
Host Human
Tissue Subglottis
Disease Keywords Subglottic stenosis, Cancer, Inflammation
Model Immortalised Line
Relevance The subglottic airway is an important gatekeeper that functions to prevent lower lung infection and is a crucial area in terms of inflammation (subglottic stenosis) and malignancy.
Production Details The SG01 Cell Line was derived from a single patients neoplasia-free human subglottic brushing specimen. The cells were immortalised using a SV40 Cell immortalization system, utilizing a recombinant lentiviral vector. SG01 were successfully lifted onto an air-liquid interface (ALI) culture system, using the appropriate growth medium. At ALI cells demonstrated cilia coverage and mucus production. Cells demonstrate tight epithelial junctions and relevant ion channels expression, including ENaC, CFTR and calcium-activated chloride channels.
Conditional No
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Adherent
Recommended Growing Conditions SG01 cells were cultured on a collagen coated container in Bronchial Epithelial Cell Growth Medium, supplemented with 100 µg/mL streptomycin and 100 U/mL penicillin at 37°C and 5% CO2. Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.05% trypsin/EDTA.

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A Human in Vitro Model of the Subglottic Airway


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References: 1 entry

A Human in Vitro Model of the Subglottic Airway


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