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VP Cell Line

Invented by Prof Fiona Watt at King's College London

Info

Catalogue Number 153454
Antigen/Gene or Protein Targets HPV transfected human keratinocytes
Parental Line Human epidermal keratinocytes - strain V
Synonyms HPV, Human papillomavirus
Host Human
Disease Keywords HPV, carincogenesis, cervical, skin
Model Immortalised Line
Relevance The paired normal and immortalised cell lines provide a good model system for studying the interaction of the HPV genome with human cells and offer the potential for further studies on the effects of carcinogenesis and oncogenes in malignant conversion of HPV16-infected keratinocytes vp HPV16-immortalised keratinocyte cell line
Production Details Primary human keratinocytes from newborn foreskin samples were used as the source of cells. These cells were then grown in culture and at passage 2 transfected with the pSV2neo/16 plasmid which contains the entire HPV16 genome.
Conditional No
Research Area Cancer, Cell Cycle, DNA Damage and Repair, Drug Discovery & Development, Immunology
Recommended Growing Conditions Composed of a 1+3 mixture of Ham’s F12 and DMEM, supplemented with 1.8x10-4M adenine, 10 % FCS, 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ng/ml EGF and 10-10M cholera toxin. Cultures should be maintained at 37oC, and medium changed every 2-3 days.

References

There are 4 reference entries for this reagent.

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References: 4 entries

Hodivala et al. 1994. Oncogene. 9(3):943-8. PMID: 8108139.

Integrin expression and function in HPV 16-immortalised human keratinocytes in the presence or absence of v-Ha-ras. Comparison with cervical intraepithelial neoplasia.

Europe PMC ID: 8108139

Pei et al. 1991. Carcinogenesis. 12(2):277-84. PMID: 1847319.

Two strains of human keratinocytes transfected with HPV16 DNA: comparison with the normal parental cells.

Europe PMC ID: 1847319


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References: 4 entries

Hodivala et al. 1994. Oncogene. 9(3):943-8. PMID: 8108139.

Integrin expression and function in HPV 16-immortalised human keratinocytes in the presence or absence of v-Ha-ras. Comparison with cervical intraepithelial neoplasia.

Pei et al. 1991. Carcinogenesis. 12(2):277-84. PMID: 1847319.

Two strains of human keratinocytes transfected with HPV16 DNA: comparison with the normal parental cells.


Add a reference