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H400 Cell Line

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Catalogue Number 153424
Antigen/Gene or Protein Targets Human oral squamous cell carcinoma, alveolar process
Tissue Alveolus
Disease Keywords Cancer
Model Tumour line
Relevance Established from a squamous cell carcinoma (SCC) of the alveolar process (20mm - 40mm) of a female patient aged 55. STNMP stage II, moderately differentiated, node negative tumour. Mutant p53, codon 283 exon 8, C to G wild type K-, N- and Ha-ras. This cell line is highly responsive to TGFbeta. These cells are non-tumourigenic on subcutaneous injection into athymic nude mice, but tumourigenic on injection into the floor of the mouth. Haplotype information: A*11,A*29; B*07,B*15; Cw*03,Cw*15
Research Area Cancer
Growth/Phenotype Keywords Adherent
Recommended Growing Conditions Split sub-confluent cultures (70-80%) 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Suggested seeding density 5 x 1000 cells/cm². Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability. DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
Cellosaurus ID CVCL_2464

References

There are 10 reference entries for this reagent.

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References: 10 entries

Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286.

Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.

Europe PMC ID: 7633286

Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893.

TGF-beta receptor regulation mediates the response to exogenous ligand but is independent of the degree of cellular differentiation in human oral keratinocytes.

Europe PMC ID: 7508893

Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.

Epidermal growth factor and transforming growth factor alpha characteristics of human oral carcinoma cell lines.

Europe PMC ID: 8286215

Yeudall et al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-7. PMID: 8180579.

Ras gene point mutation is a rare event in premalignant tissues and malignant cells and tissues from oral mucosal lesions.

Europe PMC ID: 8180579

Prime et al. 1990. J Pathol. 160(3):259-69. PMID: 1692339.

The behaviour of human oral squamous cell carcinoma in cell culture.

Europe PMC ID: 1692339


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References: 10 entries

Yeudall et al. 1995. Eur J Cancer B Oral Oncol. 31B(2):136-43. PMID: 7633286.

Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53.

Prime et al. 1994. Int J Cancer. 56(3):406-12. PMID: 7508893.

TGF-beta receptor regulation mediates the response to exogenous ligand but is independent of the degree of cellular differentiation in human oral keratinocytes.

Prime et al. 1994. Br J Cancer. 69(1):8-15. PMID: 8286215.

Epidermal growth factor and transforming growth factor alpha characteristics of human oral carcinoma cell lines.

Yeudall et al. 1993. Eur J Cancer B Oral Oncol. 29B(1):63-7. PMID: 8180579.

Ras gene point mutation is a rare event in premalignant tissues and malignant cells and tissues from oral mucosal lesions.

Prime et al. 1990. J Pathol. 160(3):259-69. PMID: 1692339.

The behaviour of human oral squamous cell carcinoma in cell culture.


Add a reference

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