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Anti-HIV gp41 [SAR1] monoclonal antibody

Invented by Nigel Dimmock at University Of Warwick

Info

Catalogue Number 153418
Applications ELISA Fn WB
Antigen/Gene or Protein Targets HIV-1 gp41 tail sequence GERDRDR
Synonyms Env antibody, Glycoprotein 41 antibody, gp41 antibody, Human Immunodeficiency Virus 1 antibody, TM antibody, Transmembrane protein antibody
Reactivity Virus
Relevance The envelope protein of human immunodeficiency virus type 1 (HIV-1) is synthesized as a gp160 polypeptide that is cleaved to form a non-covalently linked heterodimer; an external gp120 domain and a transmembrane anchoring gp41 domain. The monoclonal antibody SAR1 is a novel IgG that was raised by immunising mice with a plant virus chimera expressing the gp41 tail sequence GERDRDR. SAR1 recognizes gp41 expressed on the surface of infected cells, and binds to some, but not all, preparations of purified virions, suggesting that it may recognize non-infectious virions or degraded/immature forms of the envelope protein. SAR1 actively neutralized virus after it had attached to target cells but had little activity against free virions. Data suggest that SAR1 mediates post-attachment neutralization of virus bound to target cells by inhibiting virus-mediated fusion.
Host Mouse
Immunogen Mice were infected with Cowpea mosaic virus-HIV-1 gp41 chimera (30 g/ml) expressing the gp41 C-terminal sequence GERDRDR
Subclass IgG2a kappa
Myeloma Used Sp2/0-Ag14
Research Area Immunology, Virology

References

There are 3 reference entries for this reagent.

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References: 3 entries

Heap et al. 2005. J Gen Virol. 86(Pt 5):1499-507. PMID: 15831963.

Cheung et al. 2005. J Gen Virol. 86(Pt 1):131-8. PMID: 15604440.

Reading et al. 2003. Virology. 315(2):362-72. PMID: 14585339.


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References: 3 entries

Heap et al. 2005. J Gen Virol. 86(Pt 5):1499-507. PMID: 15831963.

Cheung et al. 2005. J Gen Virol. 86(Pt 1):131-8. PMID: 15604440.

Reading et al. 2003. Virology. 315(2):362-72. PMID: 14585339.


Add a reference

Inventor Information