Cancer Research Technology
Log in Register
Menu

Anti-p53 [DO-12]

Invented by Prof David Lane at University of Dundee

Info

Catalogue Number 153403
Applications IF WB
Antigen/Gene or Protein Targets Human TP53 / p53
Synonyms p53
Reactivity Human
Relevance Anti-p53 monoclonal antibody used to analyse and detect the ‘most mutated gene in cancer’, identifying transformed cell lines.

Background and Research Application
Anti-p53 binds p53 protein, a crucial tumour suppressor protein implicated in over 50% of cancers. p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. p53 is involved in many mechanisms of anti-cancer function, and plays a role in apoptosis, genomic stability, cell cycle regulation and inhibition of angiogenesis. p53 is known as the guardian of the genome as it functions to prevent gene mutation. Mutants of p53 which frequently occur in human cancers fail to recognise their DNA binding site, leading to the loss of tumour suppressor activity. p53 is typically found in low levels in healthy cells due to its short half-life, however in disease states it is expressed in high amounts due to somatic mutations and believed to contribute to transformation and malignancy. p53 mutations are found in over 50% of cancers with germline mutations, offering a predisposition to inherited cancer susceptibility syndrome.
Understanding the functions of mutant p53 can help in the development of new therapeutic approaches that may be useful in a broad range of cancer types.

DO-12 binds to an epitope between amino acids 250 and 270, which is located between conserved domain IV and V on an exposed turn of the protein structure. Nine isoforms of human p53 are produced by alternative splicing and promotor usage, the epitope recognised by clone DO-12 is present in all isoforms.
Host Mouse
Immunogen Recombinant human p53
Immunogen UniProt ID P04637
Subclass IgG1
Molecular Weight (kDa) 53
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
The mouse monoclonal antibody DO-12 has a weak affinity for p53 and requires a long exposure to reveal p53 isoforms.

Concentration
1mg/ml as standard
Research Area Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, Immunology

References

There are 5 reference entries for this reagent.

View All References

References: 5 entries

Pang et al. 2013. Vet J. 196(3):414-23. PMID: 23219486.

Fujita et al. 2009. Nat Cell Biol. 11(9):1135-42. PMID: 19701195.

p53 isoforms Delta133p53 and p53beta are endogenous regulators of replicative cellular senescence.

Europe PMC ID: 19701195

Bourdon et al. 2005. Genes Dev. 19(18):2122-37. PMID: 16131611.

p53 isoforms can regulate p53 transcriptional activity.

Europe PMC ID: 16131611


Add a reference

References: 5 entries

Pang et al. 2013. Vet J. 196(3):414-23. PMID: 23219486.

Fujita et al. 2009. Nat Cell Biol. 11(9):1135-42. PMID: 19701195.

p53 isoforms Delta133p53 and p53beta are endogenous regulators of replicative cellular senescence.

Bourdon et al. 2005. Genes Dev. 19(18):2122-37. PMID: 16131611.

p53 isoforms can regulate p53 transcriptional activity.


Add a reference