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Anti-PKD [P14G8]

Invented by Ayham Alnabulsi at Vertebrate Antibodies Limited

Info

Catalogue Number 153344
Applications ELISA IHC WB
Antigen/Gene or Protein Targets Tetracapsuloides bryosalmonae parasite antigen P14G8
Synonyms P14G8; T. bryosalmonae P14G8
Reactivity Tetracapsuloides bryosalmonae
Relevance Monoclonal antibody directed against P14G8 antigen of T.bryosalmonae parasite, linked to PKD.

Background and Research Application
The myxozoan parasite Tetracapsuloides bryosalmonae infects salmonid fishes. It causes proliferative kidney disease (PKD), one of the most serious parasitic diseases of salmonid populations in Europe and North America, which causes losses of up to 90% in infected populations. Currently no treatments exist to control PKD.
Anti-PKD (P14G8) targets T.bryosalmonae P14G8 antigen and is species-specific. This antibody detects the extrasporogonic stage of the parasite.
Host Mouse
Immunogen Peptide Sequence specific to P14G8 parasite antigen
Positive Control Western Blot: Infected fish kidney cells. IHC: formalin-fixed, paraffin-embedded multi tumour tissue microarray (kidney from healthy fish versus kidney from infected fish).
Subclass IgG
Myeloma Used P3X63Ag8.653
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
The P14G8 protein appears to be secreted locally and surrounds adjacent leukocytes.

This antibody is potentially neutralising and can be used for diagnostics and to study parasite-host interactions. Recommended Usage Conditions: ELISA neat; Western Blot neat; IHC neat (antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0).

Concentration
1mg/ml as standard
Research Area Aquaculture, Parasitology

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