Anti-PKD [P14G8]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
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Info
![Immunohistochemistry images are showing staining in (A) uninfected kidney tissue and (B) infected kidney using Anti-PKD [P14G8]. Arrows indicate parasite.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/9085ee17-efa4-45d0-a2c6-f57b066fa280.png)
![Western blot shows endogenous expression of (A) P14G8 protein and (B) MHCII, in trout kidney tissue using Anti-PKD [P14G8].](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/006297df-60e4-4367-bec3-92a35866f637.png)
![Immunohistochemistry images are showing staining in (A) uninfected kidney tissue and (B) infected kidney using Anti-PKD [P14G8]. Arrows indicate parasite.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/9085ee17-efa4-45d0-a2c6-f57b066fa280.png)
![Western blot shows endogenous expression of (A) P14G8 protein and (B) MHCII, in trout kidney tissue using Anti-PKD [P14G8].](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/006297df-60e4-4367-bec3-92a35866f637.png)
![Image thumbnail for Anti-PKD [P14G8]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/9085ee17-efa4-45d0-a2c6-f57b066fa280.png)
![Image thumbnail for Anti-PKD [P14G8]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/006297df-60e4-4367-bec3-92a35866f637.png)
| Catalogue Number | 153344 |
| Applications | ELISA IHC WB |
| Antigen/Gene or Protein Targets | Tetracapsuloides bryosalmonae parasite antigen P14G8 |
| Synonyms | P14G8; T. bryosalmonae P14G8 |
| Reactivity | Tetracapsuloides bryosalmonae |
| Relevance |
Monoclonal antibody directed against P14G8 antigen of T.bryosalmonae parasite, linked to PKD. Background and Research Application The myxozoan parasite Tetracapsuloides bryosalmonae infects salmonid fishes. It causes proliferative kidney disease (PKD), one of the most serious parasitic diseases of salmonid populations in Europe and North America, which causes losses of up to 90% in infected populations. Currently no treatments exist to control PKD. Anti-PKD (P14G8) targets T.bryosalmonae P14G8 antigen and is species-specific. This antibody detects the extrasporogonic stage of the parasite. |
| Host | Mouse |
| Immunogen | Peptide Sequence specific to P14G8 parasite antigen |
| Positive Control | Western Blot: Infected fish kidney cells. IHC: formalin-fixed, paraffin-embedded multi tumour tissue microarray (kidney from healthy fish versus kidney from infected fish). |
| Subclass | IgG |
| Myeloma Used | P3X63Ag8.653 |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest The P14G8 protein appears to be secreted locally and surrounds adjacent leukocytes. This antibody is potentially neutralising and can be used for diagnostics and to study parasite-host interactions. Recommended Usage Conditions: ELISA neat; Western Blot neat; IHC neat (antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0). Concentration 1mg/ml as standard |
| Research Area | Aquaculture, Parasitology |
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