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Anti-SV40T [PAB416]

Info

Catalogue Number 153338
Applications IHC IF IP WB
Antigen/Gene or Protein Targets N-terminal domain of SV40 large and small T-antigen
Reactivity Virus
Relevance Monoclonal antibody directed at large T antigen of SV40, valuable for investigation into other SV40 tumour antigens and polyomaviruses.

Background and Research Application
Anti-SV40T which binds to the large T antigen of the Simian oncogenic polyoma virus (SV40). Both the Simian virus (SV40) large T and small T antigens are encoded by the early region of the SV40 genome. The SV40 large T antigen is an early protein that interacts with the p53 and Rb tumour suppressor proteins. Large T is capable of inducing malignant transformation within cells, predominantly due to its disruption and inactivation of the 105-retinoblastoma and p53 tumour suppressor. This causes the cell to enter the S phase of the cell cycle, promoting DNA replication. Large T interacts with DNA polymerase-alpha to directly stimulate viral DNA replication.
Anti-SV40T was created in 1981 to further understand the SV40 antigens via immunochemical analysis. It was thought that detailed mapping and analysis of the SV40 tumour antigens would provide information regarding the tertiary structure and ultimately the functions of these antigens.
Host Mouse
Immunogen SV40 transformed mouse cells
Immunogen UniProt ID P03070
Positive Control SV80 or SV-T2 cells
Subclass IgG2a
Molecular Weight (kDa) 94
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions DMEM + 7.5-10% FCS
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Western blotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-5 µg antibody)

Anti-SV40T monoclonal antibody is capable of recognising JC and BK polyomaviruses however it does not bind MPCyV large T antigen. This antibody has been used in cancer research to understand the transforming properties of polyomaviruses.

Concentration
1mg/ml as standard
Research Area Virology

References

There are 19 reference entries for this reagent.

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References: 19 entries

Tanaka et al. 2018. J Vet Med Sci. 80(9):1400-1406. PMID: 30012933.

Pelletier et al. 2018. Histopathology. 73(1):162-166. PMID: 29430700.

Rigatti et al. 2016. mSphere. 1(6):. PMID: 28028546.

Toptan et al. 2016. JCI Insight. 1(2):. PMID: 27034991.

Chiaravalli et al. 2013. J Clin Pathol. 66(1):44-9. PMID: 23012397.

Sabatier et al. 2005. J Clin Pathol. 58(4):429-31. PMID: 15790713.

Immunodetection of SV40 large T antigen in human central nervous system tumours.

Europe PMC ID: 15790713

Radhakrishnan et al. 2004. J Virol. 78(13):7264-9. PMID: 15194802.

Intracellular approach for blocking JC virus gene expression by using RNA interference during viral infection.

Europe PMC ID: 15194802

Del Valle et al. 2004. J Virol. 78(7):3462-9. PMID: 15016869.

Primary central nervous system lymphoma expressing the human neurotropic polyomavirus, JC virus, genome.

Europe PMC ID: 15016869

Ricciardiello et al. 2003. Cancer Res. 63(21):7256-62. PMID: 14612521.

Induction of chromosomal instability in colonic cells by the human polyomavirus JC virus.

Europe PMC ID: 14612521

Del Valle et al. 2001. Cancer Res. 61(10):4287-93. PMID: 11358858.

Detection of JC virus DNA sequences and expression of the viral regulatory protein T-antigen in tumors of the central nervous system.

Europe PMC ID: 11358858

Masumori et al. 2001. Cancer Res. 61(5):2239-49. PMID: 11280793.

A probasin-large T antigen transgenic mouse line develops prostate adenocarcinoma and neuroendocrine carcinoma with metastatic potential.

Europe PMC ID: 11280793

Monoclonal antibodies specific for simian virus 40 tumor antigens.

Europe PMC ID: 6169844

Harlow et al. 1981. J Virol. 39(3):861-9. PMID: 6169844.


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References: 19 entries

Tanaka et al. 2018. J Vet Med Sci. 80(9):1400-1406. PMID: 30012933.

Pelletier et al. 2018. Histopathology. 73(1):162-166. PMID: 29430700.

Rigatti et al. 2016. mSphere. 1(6):. PMID: 28028546.

Toptan et al. 2016. JCI Insight. 1(2):. PMID: 27034991.

Chiaravalli et al. 2013. J Clin Pathol. 66(1):44-9. PMID: 23012397.

Sabatier et al. 2005. J Clin Pathol. 58(4):429-31. PMID: 15790713.

Immunodetection of SV40 large T antigen in human central nervous system tumours.

Radhakrishnan et al. 2004. J Virol. 78(13):7264-9. PMID: 15194802.

Intracellular approach for blocking JC virus gene expression by using RNA interference during viral infection.

Del Valle et al. 2004. J Virol. 78(7):3462-9. PMID: 15016869.

Primary central nervous system lymphoma expressing the human neurotropic polyomavirus, JC virus, genome.

Ricciardiello et al. 2003. Cancer Res. 63(21):7256-62. PMID: 14612521.

Induction of chromosomal instability in colonic cells by the human polyomavirus JC virus.

Del Valle et al. 2001. Cancer Res. 61(10):4287-93. PMID: 11358858.

Detection of JC virus DNA sequences and expression of the viral regulatory protein T-antigen in tumors of the central nervous system.

Masumori et al. 2001. Cancer Res. 61(5):2239-49. PMID: 11280793.

A probasin-large T antigen transgenic mouse line develops prostate adenocarcinoma and neuroendocrine carcinoma with metastatic potential.

Monoclonal antibodies specific for simian virus 40 tumor antigens.

Harlow et al. 1981. J Virol. 39(3):861-9. PMID: 6169844.


Add a reference

Inventor Information