Cancer Research Technology
Log in Register
Menu

HM3 Cell Line

Invented by Dr Simon Cook at Babraham Institute

Info

Catalogue Number 153328
Antigen/Gene or Protein Targets MEKK3
Parental Line HEK 293
Synonyms Mitogen-activated protein kinase kinase kinase 3, MAPK/ERK kinase kinase 3, MEK kinase 3, MEKK 3
Host Human
Tissue Kidney
Disease Keywords Cell signalling pathways; cancer; inflammation
Relevance HM3 cells are HEK 293 cells stably expressing conditional kinase ∆MEKK3:ER* from the pBabePuro plasmid. ∆MEKK3:ER* consists of the isolated kinase domain of MEKK3 fused in-frame to a modified form of the hormone binding domain of the estrogen receptor (hbER*) that can be de-repressed by 4-hydroxytamoxifen (4-HT) but not β-estradiol. In this case the * refers to a point mutation that ablates estradiol binding but allows 4-HT binding.

Activation of ∆MEKK3:ER* leads to the strong activation of the JNK and p38 pathways and a weaker activation of ERK1/2. This cell line can be used to study the role and factors impacting on the JNK, p38 and ERK1/2 signalling pathways such as gene expression, cell proliferation, cell cycle arrest and cell death.. The cells are puromycin resistant. Conditional kinase activation of ∆MEKK3:ER* can be induced with 100nM 4-HT.
Production Details HEK 293 cells were transfected with the pBabePuro plasmid expressing ∆MEKK3:ER*. Stable transfectants were selected using puromycin and ring cloning.
Conditional Yes
Conditional Description Stable transfectant, conditional functionality: MEKK3 kinase activity and ERK1/2, JNK and p38 signalling pathways activated following 4-hydroxytamoxifen (4-HT) treatment of cells.
Research Area Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, Cell Signaling & Signal Transduction, Immunology
Growth/Phenotype Keywords Adherent cell line
Recommended Growing Conditions Phenol red-free Dulbecco’s modified Eagle’s medium (DMEM) high glucose version, 2 mM L-glutamine, 10% fetal bovine serum (FBS), 2 µg/ml puromycin.
Cellosaurus ID CVCL_0306

References

There are 6 reference entries for this reagent.

View All References

References: 6 entries

Gilley et al. 2009. Cell Signal. 21(6):969-77. PMID: 19249353.

ERK1/2, but not ERK5, is necessary and sufficient for phosphorylation and activation of c-Fos.

Europe PMC ID: 19249353

Boughan et al. 2006. J Biol Chem. 281(17):11637-48. PMID: 16513653.

Nucleotide-binding oligomerization domain-1 and epidermal growth factor receptor: critical regulators of beta-defensins during Helicobacter pylori infection.

Europe PMC ID: 16513653

Todd et al. 2004. Oncogene. 23(19):3284-95. PMID: 14981547.

ERK1/2 and p38 cooperate to induce a p21CIP1-dependent G1 cell cycle arrest.

Europe PMC ID: 14981547


Add a reference

References: 6 entries

Gilley et al. 2009. Cell Signal. 21(6):969-77. PMID: 19249353.

ERK1/2, but not ERK5, is necessary and sufficient for phosphorylation and activation of c-Fos.

Boughan et al. 2006. J Biol Chem. 281(17):11637-48. PMID: 16513653.

Nucleotide-binding oligomerization domain-1 and epidermal growth factor receptor: critical regulators of beta-defensins during Helicobacter pylori infection.

Todd et al. 2004. Oncogene. 23(19):3284-95. PMID: 14981547.

ERK1/2 and p38 cooperate to induce a p21CIP1-dependent G1 cell cycle arrest.


Add a reference