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Monoclonal Antibodies

Anti-CEACAM3/5 (CD66d/e) [308/3-3] monoclonal antibody

Invented by Dr Bernhard B. Singer from LeukoCom
Invented at LeukoCom
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Image thumbnail for Anti-CEACAM3/5 (CD66d/e) [308/3-3] monoclonal antibody
Image thumbnail for Anti-CEACAM3/5 (CD66d/e) [308/3-3] monoclonal antibody
Image thumbnail for Anti-CEACAM3/5 (CD66d/e) [308/3-3] monoclonal antibody
Image thumbnail for Anti-CEACAM3/5 (CD66d/e) [308/3-3] monoclonal antibody
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Sandwich ELISA: solid phase was coated with 3 μg/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM3/5 [308/3-3] (10 μg/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.
Flow cytometry: Binding of 10 μg/ml primary positive control mAb (upper panel), Anti-CEACAM3/5 [308/3-3] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).
Immunoprecipitation of human granulocyte lysate: Utilizing 10 μg/ml Anti-CEACAM3/5 [308/3-3]. Detection of the sample using 5 μg/ml pAb Anti-CEA (Dako) followed by HRP-coupled secondary pAb. Visualized by Chemiluminescence detection.
Western blot analysis of human granulocyte lysate: Detection using 10 μg/ml Anti-CEACAM3/5 [308/3-3] followed by HRP-coupled secondary pAb. Visualized by Chemiluminescence detection.
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Image thumbnail for Anti-CEACAM3/5 (CD66d/e) [308/3-3] monoclonal antibody Image thumbnail for Anti-CEACAM3/5 (CD66d/e) [308/3-3] monoclonal antibody Image thumbnail for Anti-CEACAM3/5 (CD66d/e) [308/3-3] monoclonal antibody Image thumbnail for Anti-CEACAM3/5 (CD66d/e) [308/3-3] monoclonal antibody
Catalogue Number 153326
Applications ELISA FACS IP WB
Antigen/Gene or Protein Targets CEACAM3/5, CD66d, CD66e
Synonyms CD66d, CD66e
Reactivity Human
Relevance Monoclonal antibody directed against a shared epitope between CEACAM3 and 5.

Background and Research Application
Carcinoembryonic antigen-related cell adhesion molecule (CEACAM) 3/5 belong to the immunoglobulin superfamily. These proteins contain 1 Ig-like V-type (immunoglobulin-like) domain and the cytosolic domain is involved in S100A9 interaction. CEACAMs are reported to participate in diverse physiological processes, including cell adhesion, differentiation, proliferation, and survival, as well as in carcinogenesis and bacterial pathogenesis. CEACAM molecules play a crucial role within the innate immune system, via phagocytosis and pathogen clearance.
This antibody reacts with an antigen epitope shared by CEACAM 3 and 5.
Host Mouse
Immunogen Recombinant soluble human CEACAM3-Fc produced in HEK293 cells
Immunogen UniProt ID P40198
Subclass IgG1 kappa
Molecular Weight (kDa) 30, 180
Myeloma Used P3/NS1/1-Ag4.1
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Human CEACAM3 and CEACAM5 are two CEACAMs whose extracellular region is composed of only one V-type Ig-like extracellular domain.

Clone 308/3-3 reacts with human CEACAM3 and CEACAM5, but not human CEACAM1/4/6/7/8, rat CEACAM1, or mouse CEACAM1 & CEACAM2.

Concentration
1mg/ml as standard
Research Area Immunology

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