Cancer Research Technology
Log in Register
Menu

Anti-CEACAM8 (CD66b) [6/40c] monoclonal antibody

Invented by Dr Bernhard B. Singer at LeukoCom

Info

Catalogue Number 153323
Applications ELISA FACS IHC IP WB
Antigen/Gene or Protein Targets CEACAM8, CD66b; CD67
Synonyms CD66b; CD67
Reactivity Human
Relevance Monoclonal antibody directed against CEACAM8, used to investigate cell signalling-mediated effects on immune responses, including in cancer cells.

Background and Research Application
The carcino-embryonic antigen-related cell adhesion molecule 8 (CEACAM8) is a glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CEACAM8 is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CEACAM8, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CEACAM6. It is not expressed by peripheral blood monocytes or lymphocytes.
CEACAM8 is stored in specific vesicles of granulocytes and acts as a marker for specific vesicles for exocytosis. The soluble form of CEACAM8 binds to CEACAM1, a trans-membrane-bound molecule expressed by certain normal epithelial, endothelial, different leukocyte subpopulations and some cancer cells.
Host Mouse
Immunogen Recombinant soluble human CEACAM8-Fc produced in HEK293 cells
Immunogen UniProt ID P31997
Subclass IgG1 kappa
Molecular Weight (kDa) 100
Myeloma Used P3/NS1/1-Ag4.1
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Anti-CECAM8 6/40c does not cross-react with human CEACAM1/3/4/5/6/7, rat CECAM1, or mouse CEACAM1/2.

Concentration
1mg/ml as standard
Research Area Immunology

References

There are 4 reference entries for this reagent.

View All References

References: 4 entries

Singer et al. 2014. PLoS One. 9(4):e94106. PMID: 24743304.

Soluble CEACAM8 interacts with CEACAM1 inhibiting TLR2-triggered immune responses.

Europe PMC ID: 24743304

Klaile et al. 2013. Respir Res. 14:85. PMID: 23941132.

Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II int

Europe PMC ID: 23941132


Add a reference

References: 4 entries

Singer et al. 2014. PLoS One. 9(4):e94106. PMID: 24743304.

Soluble CEACAM8 interacts with CEACAM1 inhibiting TLR2-triggered immune responses.

Klaile et al. 2013. Respir Res. 14:85. PMID: 23941132.

Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II int


Add a reference