Anti-CEACAM8 (CD66b) [6/40c] monoclonal antibody
Invented by Dr Bernhard B. Singer from LeukoCom
Invented at LeukoCom
- Datasheet
- References (4)
- Inventor Info
Info
![Sandwich ELISA: solid phase was coated with 3 μg/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM8 [6/40c] (10 μg/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/bc103f20-6be2-46c3-aacd-31cc205012cd.jpg)
![Flow cytometry: Binding of 10 μg/ml primary positive control mAb (upper panel), Anti-CEACAM8 [6/40c] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/b3a76b35-38d1-4956-8a03-c19481a5f671.jpg)
![Immunoprecipitation of human granulocyte lysate: Utilizing 10 μg/ml Anti-CEACAM8 [6/40c]. Detection of the sample using 5 μg/ml pAb anti CEA (Dako) followed by HRP-coupled secondary pAb. Visualized by Chemiluminescence detection.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/e8ccf9e2-4660-4c9e-937b-62db8880d44d.jpg)
![Western blot analysis of human granulocyte lysate: Detection using 10 μg/ml Anti-CEACAM8 [6/40c] followed by HRP-coupled secondary pAb. Visualized by Chemiluminescence detection.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/6dfea668-a601-44c7-afc5-256081b0a6d9.jpg)
![Sandwich ELISA: solid phase was coated with 3 μg/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM8 [6/40c] (10 μg/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/bc103f20-6be2-46c3-aacd-31cc205012cd.jpg)
![Flow cytometry: Binding of 10 μg/ml primary positive control mAb (upper panel), Anti-CEACAM8 [6/40c] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/b3a76b35-38d1-4956-8a03-c19481a5f671.jpg)
![Immunoprecipitation of human granulocyte lysate: Utilizing 10 μg/ml Anti-CEACAM8 [6/40c]. Detection of the sample using 5 μg/ml pAb anti CEA (Dako) followed by HRP-coupled secondary pAb. Visualized by Chemiluminescence detection.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/e8ccf9e2-4660-4c9e-937b-62db8880d44d.jpg)
![Western blot analysis of human granulocyte lysate: Detection using 10 μg/ml Anti-CEACAM8 [6/40c] followed by HRP-coupled secondary pAb. Visualized by Chemiluminescence detection.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/6dfea668-a601-44c7-afc5-256081b0a6d9.jpg)
![Image thumbnail for Anti-CEACAM8 (CD66b) [6/40c] monoclonal antibody](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/bc103f20-6be2-46c3-aacd-31cc205012cd.jpg)
![Image thumbnail for Anti-CEACAM8 (CD66b) [6/40c] monoclonal antibody](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/b3a76b35-38d1-4956-8a03-c19481a5f671.jpg)
![Image thumbnail for Anti-CEACAM8 (CD66b) [6/40c] monoclonal antibody](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/e8ccf9e2-4660-4c9e-937b-62db8880d44d.jpg)
![Image thumbnail for Anti-CEACAM8 (CD66b) [6/40c] monoclonal antibody](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/6dfea668-a601-44c7-afc5-256081b0a6d9.jpg)
| Catalogue Number | 153323 |
| Applications | ELISA FACS IHC IP WB |
| Antigen/Gene or Protein Targets | CEACAM8, CD66b; CD67 |
| Synonyms | CD66b; CD67 |
| Reactivity | Human |
| Relevance |
Monoclonal antibody directed against CEACAM8, used to investigate cell signalling-mediated effects on immune responses, including in cancer cells. Background and Research Application The carcino-embryonic antigen-related cell adhesion molecule 8 (CEACAM8) is a glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CEACAM8 is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CEACAM8, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CEACAM6. It is not expressed by peripheral blood monocytes or lymphocytes. CEACAM8 is stored in specific vesicles of granulocytes and acts as a marker for specific vesicles for exocytosis. The soluble form of CEACAM8 binds to CEACAM1, a trans-membrane-bound molecule expressed by certain normal epithelial, endothelial, different leukocyte subpopulations and some cancer cells. |
| Host | Mouse |
| Immunogen | Recombinant soluble human CEACAM8-Fc produced in HEK293 cells |
| Immunogen UniProt ID | P31997 |
| Subclass | IgG1 kappa |
| Molecular Weight (kDa) | 100 |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Anti-CECAM8 6/40c does not cross-react with human CEACAM1/3/4/5/6/7, rat CECAM1, or mouse CEACAM1/2. Concentration 1mg/ml as standard |
| Research Area | Immunology |
References: 4 entries
Singer et al. 2014. PLoS One. 9(4):e94106. PMID: 24743304.
Soluble CEACAM8 interacts with CEACAM1 inhibiting TLR2-triggered immune responses.
Europe PMC ID: 24743304
Klaile et al. 2013. Respir Res. 14:85. PMID: 23941132.
Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II int
Europe PMC ID: 23941132
Add a reference
References: 4 entries
Singer et al. 2014. PLoS One. 9(4):e94106. PMID: 24743304.
Soluble CEACAM8 interacts with CEACAM1 inhibiting TLR2-triggered immune responses.
Klaile et al. 2013. Respir Res. 14:85. PMID: 23941132.
Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II int
Add a reference
