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Monoclonal Antibodies

Anti-Mycobacterium avium subsp. paratuberculosis [15D10]

Invented by Dr Irene Grant from Queen's University Belfast , Dr Linda Stewart from Queen's University Belfast
Invented at Queen's University Belfast
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Derived from O'Brien et al. 2016. PLoS One. 11(1):e0147870. PMID: 26815790: Competitive ELISA results. Bacterial suspension of irradiated MAP was added wells as ‘free MAP cells’ prior to the addition of the antibody. Negative control wells received assay buffer followed by the antibody. A decrease in absorbance values in the presence of ‘free MAP cells’ over negative control wells is indicative of MAP binding. Figure depicts the mean absorbance values (n = 3) ± standard error. Mean%binding inhibition was calculated based on the difference in the%binding of the antibody to the attached MAP cells in the presence and absence of ‘free MAP cells’, where negative control wells represent 100% binding.
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Image thumbnail for Anti-<i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> [15D10]
Catalogue Number 153319
Applications ELISA IP
Antigen/Gene or Protein Targets Mycobacterium avium subsp. paratuberculosis
Reactivity Mycobacterium avium
Relevance Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent for Johne’s disease, a highly-infectious wasting disease that affects a range of domestic ruminants including cattle, sheep, goats and deer. Anti-Mycobacterium avium subsp. paratuberculosis [15D10] has a high degree of specificity and has been demonstrated to work by ELISA and for immunocapture.
Host Mouse
Immunogen Ethanol-extracted surface antigens of gamma-irradiated MAP strain B4 cells
Positive Control Mycobacterium avium subsp. paratuberculosis whole cells
Subclass IgM
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM containing 10% (v/v) fetal bovine serum and 5% (v/v) penicillin/streptomycin
Strain Balb/c
Notes Direct and competitive ELISA
Specificity of immunocapture was evaluated in O'Brien et al., 2016. The study assayed three non-target Mycobacterium spp. (M. avium subsp. avium, M. bovis BCG and M. terrae). Beads coated with 6G11, the bead combination MAb 6G11 and 15D10 (50:50 mix), and dually-coated MAb 6G11 and 54.3.1AA beads demonstrated a low percentage of non-specific capture (ranging from 0.75–15% capture, with M. bovis BCG giving the highest percentage capture for all bead types).
Research Area Bacteriology

References

There are 2 reference entries for this reagent.

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References: 2 entries

O'Brien et al. 2016. PLoS One. 11(1):e0147870. PMID: 26815790.

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Novel Monoclonal Antibody and Peptide Binders for Mycobacterium avium subsp. paratuberculosis and Their Application for Magnetic Separation.

Europe PMC ID: 26815790

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References: 2 entries

O'Brien et al. 2016. PLoS One. 11(1):e0147870. PMID: 26815790.

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Novel Monoclonal Antibody and Peptide Binders for Mycobacterium avium subsp. paratuberculosis and Their Application for Magnetic Separation.

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