H9 hESC EGFP-Cone-Rod homeobox Cell Line
Invented by Prof Majlinda Lako from University of Newcastle upon Tyne , Dr Joseph Collin from University of Newcastle upon Tyne
Invented at University of Newcastle upon Tyne
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 153313 |
| Antigen/Gene or Protein Targets | Cone-Rod homeobox |
| Parental Line | H9 hESC |
| Synonyms | H9 CRX-GFP, hESC, Human embryonic stem cells, CRX,Orthodenticle Homeobox, CORD2, Cone-Rod Homeobox Protein, LCA7 3, OTX3, CRD, AMD, |
| Host | Human |
| Tissue | Embryo |
| Disease Keywords | Retinal development & age-related macular degeneration |
| Model | Reporter |
| Relevance |
A frequent cause of vision impairment and blindness associated with inherited retinal diseases and age related macular degeneration, which causes blurred or an absence of vision in the center of the visual field, is due to degeneration of retinal pigmented epithelium and photoreceptors, Replacement with stem cell derived equivalents is an excellent approach to try and preserve the retinal structures, function and vision. The Cone-Rod Homeobox gene is a key transcription factor involved in retinal development. CRX is known to be expressed in postmitotic retinal photoreceptors, and to play a key role in photoreceptor formation and maturation. This human embryonic stem cell line encodes a cone-rod homeobox gene fused at the 3' end to eGFP and has been fully characterized. This research tool enables researchers to mimic the expression of the CRX gene and to assess and investigate these cells offering pluripotent stem cell differentiation. |
| Production Details | Differentiation to 3D ocular-like structures which contain a retinal pigment epithelium, neural retina, primitive lens and corneal like structures is achieved by culture in low attachment plates with ventral neural induction media supplemented with recombinant human IGF-1 (5ng/ml) for 37 days, then in basal knockout serum free media with 10ng/ml IGF-1 until day 90 |
| Conditional | No |
| Research Area | Developmental Biology, Gene Expression, Genetic Studies Tools |
| Recommended Growing Conditions | Feeder-free mTeSR1 media, with Matrigel-coated plates |
References: 4 entries
Collin et al. 2016. Stem Cells. 34(2):311-21. PMID: 26608863.
Using Zinc Finger Nuclease Technology to Generate CRX-Reporter Human Embryonic Stem Cells as a Tool to Identify and Study the Emergence of Photoreceptors Precursors During Pluripotent Stem Cell Differentiation.
Europe PMC ID: 26608863
Mellough et al. 2015. Stem Cells. 33(8):2416-30. PMID: 25827910.
IGF-1 Signaling Plays an Important Role in the Formation of Three-Dimensional Laminated Neural Retina and Other Ocular Structures From Human Embryonic Stem Cells.
Europe PMC ID: 25827910
Add a reference
References: 4 entries
Collin et al. 2016. Stem Cells. 34(2):311-21. PMID: 26608863.
Using Zinc Finger Nuclease Technology to Generate CRX-Reporter Human Embryonic Stem Cells as a Tool to Identify and Study the Emergence of Photoreceptors Precursors During Pluripotent Stem Cell Differentiation.
Mellough et al. 2015. Stem Cells. 33(8):2416-30. PMID: 25827910.
IGF-1 Signaling Plays an Important Role in the Formation of Three-Dimensional Laminated Neural Retina and Other Ocular Structures From Human Embryonic Stem Cells.
Add a reference
