- References (2)
- Inventor Info
|Applications||ELISA FACS IHC IP WB|
|Antigen/Gene or Protein Targets||Immunoglobulin Heavy Constant Mu (IgM)|
|Synonyms||IgM, IgM heavy chain constant region antibody, Immunoglobulin mu antibody, Constant region of heavy chain of IgM antibody, Immunoglobin heavy chain mu constant region antibody|
|Reactivity||Salmon and Rainbow Trout|
Monoclonal antibody used to monitor vaccine performance in fish.
Background and Research Application
IgM is the largest antibody, and it is the first antibody to appear in the response to initial exposure to an antigen. Detection of specific antibodies in the serum of animals is an indicator of previous exposure to pathogens which is very valuable for brood stock health testing. This anti-IgM antibody provides a useful tool to monitor vaccine performance in fish and will assist in the development of future vaccines.
|Immunogen||Ammonium sulphate precipitated rainbow trout Ig fraction|
|Positive Control||ELISA fish sera; IP fish sera; FACS peripheral blood lymphocyte, spleen, kidney; Western Blot fish sera; IHC PKD infected kidney of rainbow trout.|
Purified using multi-step affinity chromatography with protein A.
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.
Points of Interest
Recommended Usage Conditions: ELISA 1/3 dilution,; Western Blot neat; IHC 1/5 dilution; IP neat; FACS neat. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).
1mg/ml as standard
There are 2 reference entries for this reagent.View All References