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Anti-IgM [BC]
RECENTLY UPDATED

Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited

Info

Catalogue Number 153308
Applications ELISA FACS IHC IP WB
Antigen/Gene or Protein Targets Immunoglobulin Heavy Constant Mu (IgM)
Synonyms IgM, IgM heavy chain constant region antibody, Immunoglobulin mu antibody, Constant region of heavy chain of IgM antibody, Immunoglobin heavy chain mu constant region antibody
Reactivity Salmon and Rainbow Trout
Relevance Monoclonal antibody used to monitor vaccine performance in fish.

Background and Research Application
IgM is the largest antibody, and it is the first antibody to appear in the response to initial exposure to an antigen. Detection of specific antibodies in the serum of animals is an indicator of previous exposure to pathogens which is very valuable for brood stock health testing. This anti-IgM antibody provides a useful tool to monitor vaccine performance in fish and will assist in the development of future vaccines.
Host Mouse
Immunogen Ammonium sulphate precipitated rainbow trout Ig fraction
Positive Control ELISA fish sera; IP fish sera; FACS peripheral blood lymphocyte, spleen, kidney; Western Blot fish sera; IHC PKD infected kidney of rainbow trout.
Subclass IgG1
Myeloma Used P3X63Ag8.653
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Recommended Usage Conditions: ELISA 1/3 dilution,; Western Blot neat; IHC 1/5 dilution; IP neat; FACS neat. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).

Concentration
1mg/ml as standard
Research Area Aquaculture

References

There are 2 reference entries for this reagent.

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References: 2 entries

Ichai et al. 2019. Curr Infect Dis Rep. 21(7):25. PMID: 31165271.

Vásquez et al. 2015. PLoS One. 10(10):e0139718. PMID: 26439739.


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References: 2 entries

Ichai et al. 2019. Curr Infect Dis Rep. 21(7):25. PMID: 31165271.

Vásquez et al. 2015. PLoS One. 10(10):e0139718. PMID: 26439739.


Add a reference