Cancer Research Technology
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Catalogue Number 153308
Applications ELISA FACS IHC IP WB
Antigen/Gene or Protein Targets Immunoglobulin Heavy Constant Mu (IgM)
Synonyms IgM, IgM heavy chain constant region antibody, Immunoglobulin mu antibody, Constant region of heavy chain of IgM antibody, Immunoglobin heavy chain mu constant region antibody
Reactivity Salmon and Rainbow Trout
Relevance Detection of specific antibodies in the serum of animals is an indicator of previous exposure to pathogens which is very valuable for broodstock health testing. Moreover, our anti-IgM antibody provides a useful tool to monitor vaccine performance in fish and will assist in the: development of future vaccines.
Host Mouse
Immunogen Ammonium sulphate precipitated rainbow trout Ig fraction
Positive Control ELISA fish sera; IP fish sera; FACS peripheral blood lymphocyte, spleen, kidney; Western Blot fish sera; IHC PKD infected kidney of rainbow trout.
Subclass IgG1
Myeloma Used P3X63Ag8.653
Strain Balb/c
Notes Recommended Usage Conditions: ELISA 1/3 dilution,; Western Blot neat; IHC 1/5 dilution; IP neat; FACS neat. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).
Research Area Aquaculture

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