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Anti-IPNV [CE4-VP2]

Invented by Ayham Alnabulsi at Vertebrate Antibodies Limited

Info

Catalogue Number 153306
Applications ELISA WB DB
Antigen/Gene or Protein Targets Infectious pancreatic necrosis virus (IPNV)
Synonyms Infectious pancreatic necrosis virus
Reactivity Virus
Relevance Monoclonal antibody to research IPNV disease of salmonid fish.

Background and Research Application
Infectious Pancreatic Necrosis Virus (IPNV) is a severe viral disease of salmonid fish. It is caused by infectious pancreatic necrosis virus, which is a member of the Birnaviridae family. This disease mainly affects young salmonids, such as trout or salmon, of less than six months, although adult fish may carry the virus without showing symptoms. IPNV is highly contagious and found worldwide, but some regions have managed to eradicate or greatly reduce the incidence of disease.
Host Mouse
Immunogen Purified IPN virus
Immunogen UniProt ID P22495
Positive Control Dot Blot, PIPNV positive tissue culture supernatants; Sandwich ELISA, suitable for use (Smail, Burnside, Watt & Munro 2003).
Subclass IgG1
Myeloma Used P3X63Ag8.653
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Antibodies are cross reactive to serotypes A2, A3 and A5 of IPNV as reported by Ross, Thomson, Melvin & Munro (1991). No cross reactivity with infectious haematopoietic necrosis virus (IHNV) and viral haemorrhagic septicemia virus (VHSV). Recommended Usage Conditions: ELISA neat; Dot Blot neat; Western blot neat.

Concentration
1mg/ml as standard
Research Area Aquaculture, Immunology, Virology

References

There are 4 reference entries for this reagent.

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References: 4 entries

Ross (1991). The use of monoclonal antibodies for detection and presumptive serotyping of infectious pancreatic necrosis virus. MSc Thesis, University of Aberdeen

Ross et al., 1991. Bulletin of the European Association of Fish Pathologists 11

Smail et al., 2003. Bulletin of the European Association of Fish Pathologists 23

Wallace et al., 2005. Bulletin of the European Association of Fish Pathologists 25.


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References: 4 entries

Ross (1991). The use of monoclonal antibodies for detection and presumptive serotyping of infectious pancreatic necrosis virus. MSc Thesis, University of Aberdeen

Ross et al., 1991. Bulletin of the European Association of Fish Pathologists 11

Smail et al., 2003. Bulletin of the European Association of Fish Pathologists 23

Wallace et al., 2005. Bulletin of the European Association of Fish Pathologists 25.


Add a reference