Anti-IgT [N5P2D2*B4]
Invented by Ayham Alnabulsi from Vertebrate Antibodies Limited
Invented at Vertebrate Antibodies Limited
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Info
![Immunohistochemistry image using Anti-IgT [N5P2D2*B4] showing positive membranous and diffuse immunostaining in infected trout kidney. Formalin-fixed paraffin-embedded tissues.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/934c2290-52c1-4c15-8799-8ef0a6638b9c.png)
![Western blot with Anti-IgT [N5P2D2*B4]. 1 = Recombinant IL-22 (negative control). 2 = Partial IgT recombinant protein (positive control).](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/852605e7-07bc-4f76-bdce-f39771232923.png)
![Immunohistochemistry image using Anti-IgT [N5P2D2*B4] showing positive membranous and diffuse immunostaining in infected trout kidney. Formalin-fixed paraffin-embedded tissues.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/934c2290-52c1-4c15-8799-8ef0a6638b9c.png)
![Western blot with Anti-IgT [N5P2D2*B4]. 1 = Recombinant IL-22 (negative control). 2 = Partial IgT recombinant protein (positive control).](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/852605e7-07bc-4f76-bdce-f39771232923.png)
![Image thumbnail for Anti-IgT [N5P2D2*B4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/934c2290-52c1-4c15-8799-8ef0a6638b9c.png)
![Image thumbnail for Anti-IgT [N5P2D2*B4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/852605e7-07bc-4f76-bdce-f39771232923.png)
| Catalogue Number | 153304 |
| Applications | ELISA FACS IHC WB |
| Antigen/Gene or Protein Targets | IgT, Immunoglobulin Heavy Constant Tau |
| Synonyms | IgT |
| Reactivity | Salmon and Rainbow Trout |
| Relevance | The IgT isotype, which is unique for teleosts, appears to be specialised to mucosal immune responses. |
| Host | Mouse |
| Immunogen | Ovalbumin-conjugated synthetic peptides- N5 peptide |
| Positive Control | ELISA, immunized fish sera; Western Blot, recombinant IgT, immunized fish sera; FACS, head kindney; IHC, Formalin-fixed, paraffin-embedded PKD infected fish kidney. |
| Subclass | IgG1 |
| Myeloma Used | P3X63Ag8.653 |
| Strain | Balb/c |
| Notes | Recommended Usage Conditions: ELISA neat; Western blot neat; FACS 1/2 dilution; IHC 1/5 dilution, antigen retrieval: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0 |
| Research Area | Aquaculture |
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